AMPK (G1/B1/A2) Heterotrimer Cell Lysate | PRKAG1 cell lysate
Human AMPK (G1/B1/A2) Heterotrimer Baculovirus-Insect cells Overexpression Lysate
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Samples are stable for up to twelve months from date of receipt.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Uniprot Description
AMPKG1: a non-catalytic subunit of AMPK, a highly conserved kinase of the CAMKL family. The holoenzyme consists of a catalytic subunit (alpha) and two regulatory subunits (beta, gamma). Each subunit exists as multiple genetic isoforms giving rise to 12 different possible combinations of holoenzyme. Plays a key role in regulation of energy homeostasis. Activated by an elevated AMP-ATP ratio due to environmental stress, such as heat shock, hypoxia and ischemia. AMPK phosphorylates and regulates many metabolic enzymes.
Protein type: Protein kinase, regulatory subunit; Autophagy
Chromosomal Location of Human Ortholog: 12q12-q14
Cellular Component: membrane; nucleus; cytosol; AMP-activated protein kinase complex
Molecular Function: AMP-activated protein kinase activity; protein binding; cAMP-dependent protein kinase activity; cAMP-dependent protein kinase regulator activity; ADP binding; protein kinase binding; ATP binding; protein kinase activity; AMP binding
Biological Process: mitochondrion organization and biogenesis; protein heterooligomerization; organelle organization and biogenesis; positive regulation of protein kinase activity; regulation of glycolysis; insulin receptor signaling pathway; spermatogenesis; cell cycle arrest; signal transduction; protein amino acid phosphorylation; fatty acid biosynthetic process
Research Articles on PRKAG1
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Product Notes
The PRKAG1 prkag1 (Catalog #AAA8118062) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AMPK (G1/B1/A2) Heterotrimer can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the PRKAG1 prkag1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Pro33 1, Met1-Ile27 0, Met1-Arg55 2. It is sometimes possible for the material contained within the vial of "AMPK (G1/B1/A2) Heterotrimer, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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