Prostaglandin D2 Synthase Cell Lysate | PTGDS cell lysate
Mouse Prostaglandin D2 Synthase HEK293 Overexpression Lysate
Gene Names
Ptgds; PGD2; PGDS; 21kDa; PGDS2; Ptgs3; L-PGDS
Applications
Western Blot
Synonyms
Prostaglandin D2 Synthase; Mouse Prostaglandin D2 Synthase HEK293 Overexpression Lysate; Mouse PTGDS/L-PGDS HEK293 Cell Lysate (WB positive control); prostaglandin D2 synthase 21kDa (brain); Mouse 21kDa Overexpression Lysate; Mouse L-PGDS Overexpression Lysate; Mouse PGD2 Overexpression Lysate; Mouse PGDS Overexpression Lysate; Mouse PGDS2 Overexpression Lysate; Mouse Ptgs3 Overexpression Lysate; PTGDS cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
176aa
Sequence
Met1-Glu189
Applicable Applications for PTGDS cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse Ptgds (NP_032989.2) (Met1-Glu189) was expressed with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for PTGDS cell lysate
PTGDS, also known as L-PGDS, belongs to the calycin superfamily, lipocalin family. Lipocalins share limited regions of sequence homology and a common tertiary structure architecture. They transport small hydrophobic molecules such as steroids, bilins, retinoids, and lipids. PTGDS is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of PGH2 to PGD2. It is involved in smooth muscle contraction/relaxation and a variety of central nervous system functions. PTGDS may have an anti-apoptotic role in oligodendrocytes. It binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.
This Mouse Prostaglandin D2 Synthase overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Prostaglandin D2 Synthase protein from the overexpression lysate was verified.
This Mouse Prostaglandin D2 Synthase overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Prostaglandin D2 Synthase protein from the overexpression lysate was verified.
Product Categories/Family for PTGDS cell lysate
References
Aebersold R, et al. (1993) Identification of a brain-specific human cerebrospinal fluid glycoprotein, beta-trace protein. Theor Electrophor. 3:229-234.Oliver K, et al. (2004) DNA sequence and analysis of human chromosome 9. Nature. 429:369-374.Bonaldo MF, et al. (1997) Normalization and subtraction: two approaches to facilitate gene discovery. Genome Res. 6(9):791-806.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,941 Da
NCBI Official Full Name
prostaglandin-H2 D-isomerase
NCBI Official Synonym Full Names
prostaglandin D2 synthase (brain)
NCBI Official Symbol
Ptgds
NCBI Official Synonym Symbols
PGD2; PGDS; 21kDa; PGDS2; Ptgs3; L-PGDS
NCBI Protein Information
prostaglandin-H2 D-isomerase
Protein Family
Research Articles on PTGDS
Similar Products
Product Notes
The PTGDS (Catalog #AAA8117136) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 176aa. AAA Biotech's Prostaglandin D2 Synthase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the PTGDS for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Glu18 9. It is sometimes possible for the material contained within the vial of "Prostaglandin D2 Synthase, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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