IL20RB cell lysate
Human IL20RB Baculovirus-Insect Overexpression Lysate
Gene Names
IL20RB; DIRS1; FNDC6; IL-20R2
Applications
Western Blot
Synonyms
IL20RB; Human IL20RB Baculovirus-Insect Overexpression Lysate; Human IL20RB Insect Cell Lysate (WB positive control); interleukin 20 receptor beta; Human DIRS1 Overexpression Lysate; Human FNDC6 Overexpression Lysate; Human IL-20R2 Overexpression Lysate; IL20RB cell lysate
Host
Baculovirus-Insect Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
212aa
Sequence
Met1-Ala230
Applicable Applications for IL20RB cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.
3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.
Sequence Construction
A DNA sequence encoding the human IL20RB (NP_653318.2) (Met1-Ala230) was expressed with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for IL20RB cell lysate
IL20RB belongs to the type II cytokine receptor family. There are two kinds of type II cytokine receptors: cytokine receptors that bind type I and type II interferons; cytokine receptors that bind members of the interleukin-10 family (interleukin-10, interleukin-20, and interleukin-22). Type II cytokine receptors are similar to type I cytokine receptors except they do not possess the signature sequence WSXWS that is characteristic of type I receptors. They are expressed on the surface of certain cells, which bind and respond to a select group of cytokines. These receptors are related predominantly by sequence similarities in their extracellular portions that are composed of tandem Ig-like domains. The intracellular domain of type II cytokine receptors is typically associated with a tyrosine kinase belonging to the Janus kinase (JAK) family. IL20RB and IL20RA form a heterodimeric receptor for interleukin-20.
This Human IL20RB overexpression lysate was created in Baculovirus-Insect Cells and intented for use as a Western blot (WB) positive control. Purification of IL20RB protein from the overexpression lysate was verified.
This Human IL20RB overexpression lysate was created in Baculovirus-Insect Cells and intented for use as a Western blot (WB) positive control. Purification of IL20RB protein from the overexpression lysate was verified.
Product Categories/Family for IL20RB cell lysate
References
Zhu H, et al. (2009) Expression pattern of mda-7/IL-24 receptors in liver cancer cell lines. Hepatobiliary Pancreat Dis Int. 8(4):402-6.Logsdon NJ, et al. (2012) Purification, crystallization and preliminary X-ray diffraction analysis of the IL-20-IL-20R1-IL-20R2 complex. Acta Crystallogr Sect F Struct Biol Cryst Commun. 68(Pt 1):89-92.Kingo K, et al. (2008) Association analysis of IL20RA and IL20RB genes in psoriasis. Genes Immun. 9(5):445-51.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,076 Da
NCBI Official Full Name
interleukin-20 receptor subunit beta
NCBI Official Synonym Full Names
interleukin 20 receptor beta
NCBI Official Symbol
IL20RB
NCBI Official Synonym Symbols
DIRS1; FNDC6; IL-20R2
NCBI Protein Information
interleukin-20 receptor subunit beta; IL-20RB; IL-20R-beta; interleukin-20 receptor II; IL-20 receptor subunit beta; fibronectin type III domain containing 6
UniProt Protein Name
Interleukin-20 receptor subunit beta
Protein Family
UniProt Gene Name
IL20RB
UniProt Synonym Gene Names
DIRS1; IL-20 receptor subunit beta; IL-20R-beta; IL-20RB
UniProt Entry Name
I20RB_HUMAN
NCBI Description
IL20RB and IL20RA (MIM 605620) form a heterodimeric receptor for interleukin-20 (IL20; MIM 605619) (Blumberg et al., 2001 [PubMed 11163236]).[supplied by OMIM, Feb 2009]
Uniprot Description
IL20RB: The IL20RA/IL20RB dimer is a receptor for IL19, IL20 and IL24. The IL22RA1/IL20RB dimer is a receptor for IL20 and IL24. Belongs to the type II cytokine receptor family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q22.3
Cellular Component: integral to membrane; plasma membrane
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-20 binding
Biological Process: positive regulation of interleukin-4 production; negative regulation of T cell proliferation; inflammatory response to antigenic stimulus; homeostasis of number of cells within a tissue; immune response-inhibiting signal transduction; negative regulation of interferon-gamma production; cytokine and chemokine mediated signaling pathway; negative regulation of type IV hypersensitivity; positive regulation of interleukin-10 production; negative regulation of interleukin-2 production
Research Articles on IL20RB
Similar Products
Product Notes
The IL20RB il20rb (Catalog #AAA8115728) is a Cell Lysate produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 212aa. AAA Biotech's IL20RB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the IL20RB il20rb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ala23 0. It is sometimes possible for the material contained within the vial of "IL20RB, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
