RYBP cell lysate
Human RYBP Baculovirus-Insect cells Overexpression Lysate
Gene Names
RYBP; AAP1; DEDAF; YEAF1
Applications
Western Blot
Synonyms
RYBP; Human RYBP Baculovirus-Insect cells Overexpression Lysate; Human APAP-1/AAP1/RYBP Insect Cell Lysate (WB positive control); RING1 and YY1 binding protein; Human AAP1 Overexpression Lysate; Human DEDAF Overexpression Lysate; Human YEAF1 Overexpression Lysate; RYBP cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
465aa
Sequence
Met1-Phe228
Applicable Applications for RYBP cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human RYBP (Q8N488) (Met1-Phe228) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for RYBP cell lysate
APAP-1, also known as AAP1 and RYBP, is widely expressed. It is highest expressed in lymphoid tissues and placenta. APAP-1 contains 1 RanBP2-type zinc finger. It may bind to DNA. APAP-1 inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. It may be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. APAP-1 also promotes apoptosis.
This Human RYBP overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of RYBP protein from the overexpression lysate was verified.
This Human RYBP overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of RYBP protein from the overexpression lysate was verified.
References
Li Mao, et al. (2009) RYBP stabilizes p53 by modulating MDM2. EMBO Rep. 10(2):166-72. Schlisio, et al. (2002) Interaction of YY1 with E2Fs, mediated by RYBP, provides a mechanism for specificity of E2F function. EMBO J. 21(21):5775-86. Peter M E, et al. (2001) The death effector domain-associated factor plays distinct regulatory roles in the nucleus and cytoplasm. J Biol Chem. 276(34):31945-52.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,822 Da
NCBI Official Full Name
RING1 and YY1-binding protein
NCBI Official Synonym Full Names
RING1 and YY1 binding protein
NCBI Official Symbol
RYBP
NCBI Official Synonym Symbols
AAP1; DEDAF; YEAF1
NCBI Protein Information
RING1 and YY1-binding protein; APAP-1; DED-associated factor; YY1 and E4TF1 associated factor 1; YY1 and E4TF1-associated factor 1; apoptin-associating protein 1; death effector domain-associated factor; ring1 interactor RYBP
UniProt Protein Name
RING1 and YY1-binding protein
Protein Family
UniProt Gene Name
RYBP
UniProt Synonym Gene Names
DEDAF; YEAF1; APAP-1; DED-associated factor
UniProt Entry Name
RYBP_HUMAN
Uniprot Description
RYBP: Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 3p13
Cellular Component: nucleoplasm; cytoplasm; PcG protein complex
Molecular Function: protein binding; DNA binding; zinc ion binding; transcription corepressor activity
Biological Process: transcription, DNA-dependent; apoptosis; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter
Research Articles on RYBP
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Product Notes
The RYBP rybp (Catalog #AAA8115364) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 465aa. AAA Biotech's RYBP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the RYBP rybp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Phe22 8. It is sometimes possible for the material contained within the vial of "RYBP, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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