SHISA3 cell lysate
Human SHISA3 HEK293 Overexpression Lysate
Gene Names
SHISA3; hShisa3
Applications
Western Blot
Synonyms
SHISA3; Human SHISA3 HEK293 Overexpression Lysate; Human SHISA3 HEK293 Cell Lysate (WB positive control); shisa family member 3; Human hShisa3 Overexpression Lysate; SHISA3 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
311aa
Sequence
Met1-Pro98
Applicable Applications for SHISA3 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human SHISA3 (AAI27691.1) (Met1-Pro98) was expressed with the Fc region of mouse IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for SHISA3 cell lysate
SHISA3(high)CLL are characterized by a restrained activation of Wnt signaling and sensibility to lenalidomide-induced apoptosis. SHISA3 promoter hypermethylation might increase the risk of LSCC through regulation of gene expression and is a potential and prognostic biomarker for LSCC. Shisa3 is a novel tumor suppressor identified in lung cancer. SHISA3 gene is epigenetically inactivated in a substantial fraction of CRC, and its hypermethylation is of prognostic significance in predicting clinical outcome.
This Human SHISA3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SHISA3 protein from the overexpression lysate was verified.
This Human SHISA3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SHISA3 protein from the overexpression lysate was verified.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,832 Da
NCBI Official Full Name
Shisa homolog 3 (Xenopus laevis)
NCBI Official Synonym Full Names
shisa family member 3
NCBI Official Symbol
SHISA3
NCBI Official Synonym Symbols
hShisa3
NCBI Protein Information
protein shisa-3 homolog
Protein Family
Research Articles on SHISA3
Similar Products
Product Notes
The SHISA3 (Catalog #AAA8115205) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 311aa. AAA Biotech's SHISA3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SHISA3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Pro98. It is sometimes possible for the material contained within the vial of "SHISA3, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
