AK3L1 Cell Lysate | AK4 cell lysate

Human AK3L1 Baculovirus-Insect cells Overexpression Lysate

Gene Names

AK4; AK3; AK 4; AK3L1; AK3L2

Applications

Western Blot

Synonyms

AK3L1; Human AK3L1 Baculovirus-Insect cells Overexpression Lysate; Human AK4/Adenylate Kinase 4/AK3L1 Insect Cell Lysate (WB positive control); adenylate kinase 4; Human AK3 Overexpression Lysate; Human AK3L1 Overexpression Lysate; Human AK3L2 Overexpression Lysate; Human AK4 Overexpression Lysate; AK4 cell lysate

Ordering
Cat. Num. AAA8114958 For Research Use Only!

Host

Baculovirus-Insect cells

Form/Format

1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Sequence Positions

460aa

Sequence

Ala2-Tyr223

Applicable Applications for AK4 cell lysate

Western Blot (WB)

Application Notes

WB: Use at an assay dependent dilution.

Species

Human

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.

Sequence Construction

A DNA sequence encoding the human AK4 (P27144-1) (Ala 2-Tyr 223) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.

Related Product Information for AK4 cell lysate

Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member theadenylate kinase family. AK4/AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4/AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 (AK4/AK3L1) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4/AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4/AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4/AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously.

This Human AK3L1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of AK3L1 protein from the overexpression lysate was verified.

References

Xu G., et al., (1992), Characterization of human adenylate kinase 3 (AK3) cDNA and mapping of the AK3 pseudogene to an intron of the NF1 gene. Genomics 13:537-542.Gregory S.G., et al., (2006), The DNA sequence and biological annotation of human chromosome 1.Nature 441:315-321.Ota T., et al., (2004), Complete sequencing and characterization of 21, 243 full-length human cDNAs.Nat. Genet. 36:40-45.

NCBI and Uniprot Product Information

NCBI GI #

125157

NCBI GeneID

205

UniProt Accession #

P27144; Q6IBH4; Q6NXQ5; Q8IUU9; B2R927; D3DQ62

Molecular Weight

223

NCBI Official Full Name

Adenylate kinase 4, mitochondrial

NCBI Official Synonym Full Names

adenylate kinase 4

NCBI Official Symbol

AK4

NCBI Official Synonym Symbols

AK3; AK 4; AK3L1; AK3L2

NCBI Protein Information

adenylate kinase 4, mitochondrial; AK4; AK 4; adenylate kinase 3-like 1; ATP-AMP transphosphorylase; mitochondrial adenylate kinase-3; nucleoside-triphosphate-adenylate kinase; adenylate kinase isoenzyme 4, mitochondrial; GTP:AMP phosphotransferase AK4, m

UniProt Protein Name

Adenylate kinase 4, mitochondrial

UniProt Gene Name

AK4

UniProt Entry Name

KAD4_HUMAN

NCBI Description

This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq, Jul 2008]

Uniprot Description

AK3L1: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. May also be active with GTP. Belongs to the adenylate kinase family.

Protein type: Nucleotide Metabolism - purine; EC 2.7.4.10; EC 2.7.4.6; Mitochondrial; Kinase, other

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: GTP binding; nucleoside diphosphate kinase activity; adenylate kinase activity; nucleoside triphosphate adenylate kinase activity; ATP binding

Biological Process: response to drug; ATP metabolic process; nucleoside triphosphate biosynthetic process; nucleoside diphosphate phosphorylation; ADP biosynthetic process; brain development; liver development; AMP metabolic process; GTP metabolic process

Research Articles on AK4

1. a stress-protective function of AK4

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Product Notes
The AK4 ak4 (Catalog #AAA8114958) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 460aa. AAA Biotech's AK3L1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the AK4 ak4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Ala2-Tyr22 3. It is sometimes possible for the material contained within the vial of "AK3L1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.
Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.