CD200RLa Cell Lysate | CD200R1L cell lysate
Human CD200RLa HEK293 Overexpression Lysate
Applications
Western Blot
Synonyms
CD200RLa; Human CD200RLa HEK293 Overexpression Lysate; Human CD200R1L/CD200R2/CD200RLa HEK293 Cell Lysate (WB positive control); CD200 receptor 1 like; Human CD200R2 Overexpression Lysate; Human CD200RLa Overexpression Lysate; CD200R1L cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
227aa
Sequence
Met1-Leu239
Applicable Applications for CD200R1L cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human CD200R1L (AAT00538.1) extracellular domain (Met1-Leu239) was expressed, fused with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CD200R1L cell lysate
Cell surface glycoprotein CD2 receptor 2, also known as Cell surface glycoprotein CD2 receptor 1-like, Cell surface glycoprotein OX2 receptor 2, CD2 receptor-like 2, CD2Rla, CD2R1L and CD2R2, is a single-pass type I membrane protein which belongs to theCD2R family. CD2R1L/CD2R2. It contains oneIg-like C2-type (immunoglobulin-like) domain and oneIg-like V-type (immunoglobulin-like) domain. CD2 is a transmembrane protein delivering immunoregulatory signals after engagement of CD2R. A family of CD2Rs exist (CD2R1, CD2R2, CD2R3, CD2R4) with different tissue expression and functional activity. In the presence of anti-CD2R2/CD2R3 monoclonal antibodies (mAbs), bone-marrow cells cultured in the presence of (interleukin [IL]-4+granulocyte-macrophage colony-stimulating factor) differentiate into dendritic cells (DCs), which induce CD4+CD25+ Treg. Interaction between the relatively ubiquitously expressed molecule CD2 and one of its receptors, CD2R1, resulted in direct suppression of alloreactivity, engagement of alternate receptors led instead to altered differentiation of dendritic cells (DCs) from marrow precursors, which could in turn foster development of Foxp3(+) regulatory T cells. Unlike anti-CD2R1, anti-CD2R2 both promotes development of DCs with capacity to induce Treg and directly augments thymocyte production of Treg.
This Human CD200RLa overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD200RLa protein from the overexpression lysate was verified.
This Human CD200RLa overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CD200RLa protein from the overexpression lysate was verified.
References
Wright G.J., et al., (2003), Characterization of the CD200 receptor family in mice and humans and their interactions with CD200. J. Immunol. 171:3034-3046.Gorczynski R.M., et al., (2004), Structural and functional heterogeneity in the CD200R family of immunoregulatory molecules and their expression at the feto-maternal interface.Am. J. Reprod. Immunol. 52:147-163.Muzny D.M., et al., (2006), The DNA sequence, annotation and analysis of human chromosome 3.Nature 440:1194-1198.
NCBI and Uniprot Product Information
Molecular Weight
The recombinant human CD200R1L consists of 227 amino acids and predictes a molecular mass of 25.2kDa.
In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CD200R1L is approximately 45-60kDa due to glycosylation.
In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CD200R1L is approximately 45-60kDa due to glycosylation.
Protein Family
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Product Notes
The CD200R1L (Catalog #AAA8114612) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 227aa. AAA Biotech's CD200RLa can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CD200R1L for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Leu23 9. It is sometimes possible for the material contained within the vial of "CD200RLa, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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