B3GNT2 cell lysate
Human B3GNT2 HEK293 Overexpression Lysate
Gene Names
B3GNT2; B3GNT; BGNT2; B3GNT1; BGnT-2; beta-1; 3-Gn-T1; 3-Gn-T2; B3GN-T2; B3GNT-2; BETA3GNT; beta3Gn-T1; beta3Gn-T2
Applications
Western Blot
Synonyms
B3GNT2; Human B3GNT2 HEK293 Overexpression Lysate; Human B3GNT2 HEK293 Cell Lysate (WB positive control); UDP-GlcNAc:betaGal beta-1; 3-N-acetylglucosaminyltransferase 2; Human B3GN-T2 Overexpression Lysate; Human B3GNT Overexpression Lysate; Human B3GNT-2 Overexpression Lysate; Human B3GNT1 Overexpression Lysate; Human BETA3GNT Overexpression Lysate; Human BGnT-2 Overexpression Lysate; Human BGNT2 Overexpression Lysate; B3GNT2 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
629aa
Sequence
Lys29-Cys397
Applicable Applications for B3GNT2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human B3GNT2 (Q9NY97-1) (Lys29-Cys397) was expressed, with the fused Fc region of human IgG1 at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for B3GNT2 cell lysate
B3GNT2 belongs to the beta-1, 3-N-acetylglucosaminyltransferase family. It is a type II transmembrane protein that prefers the substrate of lacto-N-neotetraose. Alternative splicing produced 2 isoforms of the human protein. B3GNT2 catalyzes the initiation and elongation of poly-N-acetyllactosamine chains. Enzymatic activities of some glycosyltransferases are markedly increased via complex formation with other transferases or cofactor proteins. B3GNT2 and beta3Gn-T8 can form a heterodimer in vitro and that the complex exhibits much higher enzymatic activity than either enzyme alone. It is found that up-regulation of beta3Gn-T8 in differentiated HL-60 cells may increase poly-N-acetyllactosamine chains by activating intrinsic B3GNT2.
This Human B3GNT2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of B3GNT2 protein from the overexpression lysate was verified.
This Human B3GNT2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of B3GNT2 protein from the overexpression lysate was verified.
References
Australo-An, et al. (2010) Genome-wide association study of ankylosing spondylitis identifies non-MHC susceptibility loci. Nat Genet. 42(2):123-7.Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40.Seko A, et al. (2008) Activation of beta1, 3-N-acetylglucosaminyltransferase-2 (beta3Gn-T2) by beta3Gn-T8. Possible involvement of beta3Gn-T8 in increasing poly-N-acetyllactosamine chains in differentiated HL-60 cells. J Biol Chem. 283(48):33094-100.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,484 Da
NCBI Official Full Name
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2
NCBI Official Synonym Full Names
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
NCBI Official Symbol
B3GNT2
NCBI Official Synonym Symbols
B3GNT; BGNT2; B3GNT1; BGnT-2; beta-1; 3-Gn-T1; 3-Gn-T2; B3GN-T2; B3GNT-2; BETA3GNT; beta3Gn-T1; beta3Gn-T2
NCBI Protein Information
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2
UniProt Protein Name
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2
UniProt Gene Name
B3GNT2
UniProt Synonym Gene Names
B3GALT7; B3GNT1; BGnT-1; Beta-1,3-Gn-T1; Beta3Gn-T1; Beta-1,3-GalTase 7; Beta3Gal-T7; Beta3GalT7; b3Gal-T7; BGnT-2; Beta-1,3-Gn-T2; Beta-1,3-N-acetylglucosaminyltransferase 2; Beta3Gn-T2
NCBI Description
This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It prefers the substrate of lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2016]
Uniprot Description
B3GNT2: Catalyzes the initiation and elongation of poly-N- acetyllactosamine chains. Belongs to the glycosyltransferase 31 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell development/differentiation; EC 2.4.1.-; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Glycan Metabolism - keratan sulfate biosynthesis; Membrane protein, integral; Motility/polarity/chemotaxis; Transferase
Chromosomal Location of Human Ortholog: 2p15
Cellular Component: Golgi membrane
Molecular Function: N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity
Biological Process: keratan sulfate biosynthetic process; O-glycan processing; poly-N-acetyllactosamine biosynthetic process
Research Articles on B3GNT2
Similar Products
Product Notes
The B3GNT2 b3gnt2 (Catalog #AAA8114540) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 629aa. AAA Biotech's B3GNT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the B3GNT2 b3gnt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Lys29-Cys3 97. It is sometimes possible for the material contained within the vial of "B3GNT2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
