BMPRIB Cell Lysate | BMPR1B cell lysate
Human BMPRIB Baculovirus-Insect cells Overexpression Lysate
Gene Names
BMPR1B; ALK6; AMDD; BDA2; ALK-6; BDA1D; CDw293
Applications
Western Blot
Synonyms
BMPRIB; Human BMPRIB Baculovirus-Insect cells Overexpression Lysate; Human BMPR1B/ALK-6 (149-502) Insect Cell Lysate (WB positive control); bone morphogenetic protein receptor; type IB; Human ALK-6 Overexpression Lysate; Human ALK6 Overexpression Lysate; Human CDw293 Overexpression Lysate; BMPR1B cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
591aa
Sequence
Arg149-Leu502
Applicable Applications for BMPR1B cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human ALK6 (NP_001194.1) cytoplasmic domain (Arg 149-Leu 502) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for BMPR1B cell lysate
BMPR1B(bone morphogenetic protein receptor, type IB), also known as ALK6, is a member of the bone morphogenetic protein (BMP) receptor family. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. BMPR1B is the major transducer of signals in precartilaginous condensations as demonstrated in experiments using constitutively active BMPR1B receptors. BMPR1B is a more effective transducer of GDF5 than BMPR1A. Unlike BMPR1A null mice, which die at an early embryonic stage, BMPR1B null mice are viable.
This Human BMPRIB overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of BMPRIB protein from the overexpression lysate was verified.
This Human BMPRIB overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of BMPRIB protein from the overexpression lysate was verified.
References
Ide H, et al. (1998) Assignment of the BMPR1A and BMPR1B genes to human chromosome 10q22.3 and 4q23--q24 byin situ hybridization and radiation hybrid map ping. Cytogenet. Cell Genet. 81(3-4): 285-6.Mishina Y, et al. (2004) Bone morphogenetic protein type IA receptor signaling regulates postnatal osteoblast function and bone remodeling. J Biol Chem. 279(26): 27560-6.Yoon BS, et al. (2005) Bmpr1a and Bmpr1b have overlapping functions and are essential for chondrogenesis in vivo. Proc Natl Acad Sci. 102(14): 5062-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,582 Da
NCBI Official Full Name
bone morphogenetic protein receptor type-1B isoform b
NCBI Official Synonym Full Names
bone morphogenetic protein receptor type 1B
NCBI Official Symbol
BMPR1B
NCBI Official Synonym Symbols
ALK6; AMDD; BDA2; ALK-6; BDA1D; CDw293
NCBI Protein Information
bone morphogenetic protein receptor type-1B
UniProt Protein Name
Bone morphogenetic protein receptor type-1B
Protein Family
UniProt Gene Name
BMPR1B
UniProt Synonym Gene Names
BMP type-1B receptor; BMPR-1B
UniProt Entry Name
BMR1B_HUMAN
Uniprot Description
BMPR1B: a kinase of the STKR family. A type I cell-surface receptor for bone morphogenetic protein. Expressed at ighest level in the prostate. May be upregulated by androgen stimulation in cells which expresses the androgen receptor.
Protein type: Protein kinase, Ser/Thr (receptor); Kinase, protein; EC 2.7.11.30; Membrane protein, integral; Protein kinase, TKL; TKL group; STKR family; Type1 subfamily
Chromosomal Location of Human Ortholog: 4q22-q24
Cellular Component: integral to plasma membrane; plasma membrane; receptor complex
Molecular Function: protein serine/threonine kinase activity; protein binding; transforming growth factor beta receptor activity, type I; metal ion binding; SMAD binding; ATP binding; transmembrane receptor protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: chondrocyte development; positive regulation of bone mineralization; protein amino acid phosphorylation; limb morphogenesis; BMP signaling pathway; positive regulation of osteoblast differentiation; ovulation cycle; dorsal/ventral pattern formation; eye development; retina development in camera-type eye; proteoglycan biosynthetic process; ovarian cumulus expansion; inflammatory response; positive regulation of cell differentiation; skeletal development; cartilage condensation; retinal ganglion cell axon guidance
Disease: Brachydactyly, Type A2; Chondrodysplasia, Acromesomelic, With Genital Anomalies
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Product Notes
The BMPR1B bmpr1b (Catalog #AAA8113875) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 591aa. AAA Biotech's BMPRIB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the BMPR1B bmpr1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Arg149-Leu 502. It is sometimes possible for the material contained within the vial of "BMPRIB, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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