Rabbit CD3D & CD3E Heterodimer Monoclonal Antibody | anti-CD3D antibody

Recombinant Anti-CD3D & CD3E Heterodimer Antibody, Rabbit Monoclonal

Cat. Num. AAA8113340

• Gene Names: CD3D; T3D; IMD19; CD3-DELTA
• Reactivity: Human, Cynomolgus
• Applications: ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: CD3D & CD3E Heterodimer; Monoclonal Antibody; Recombinant Anti-CD3D & CD3E Heterodimer Antibody; Rabbit Monoclonal; CD3D & CD3E Antibody; Rabbit MAb; CD3d molecule; delta (CD3-TCR complex); Anti-CD3-DELTA Antibody; Anti-IMD19 Antibody; Anti-T3D Antibody; anti-CD3D antibody

• Host: Rabbit
• Reactivity: Human, Cynomolgus
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 301
• Specificity: Human CD3D & CD3E Heterodimer.; Has cross-reactivity in ELISA and IHC with Human CD3e/CD3 epsilon Protein.; No cross-reactivity in ELISA and IHC with Human CD3d/CD3 delta Protein
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CD3D antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM)
• Application Notes: ELISA: 1:5000-1:10,000; IHC-P: 1:100-1:500; FC/FACS/FCM: 1:25-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human CD3D & CD3E Heterodimer protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD3D & CD3E Heterodimer.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining CD3E in human tonsil with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human CD3E in cynomolgus macaque tonsil with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human CD3E in cynomolgus macaque lymphnode with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human CD3E expression on human whole blood lymphocytes. Cells were stained with purified anti-Human CD3E, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)

Immunohistochemistry (IHC)

(Immunochemical staining CD3 in cynomolgus spleen with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining CD3 in cynomolgus lymph node with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

NCBI and Uniprot Product Information

• NCBI GI #: 115985
• NCBI GeneID: 915
• UniProt Accession #: P04234; A8MVP6
• Molecular Weight: 171
• NCBI Official Full Name: T-cell surface glycoprotein CD3 delta chain
• NCBI Official Synonym Full Names: CD3d molecule, delta (CD3-TCR complex)
• NCBI Official Symbol: CD3D
• NCBI Official Synonym Symbols: T3D; IMD19; CD3-DELTA
• NCBI Protein Information: T-cell surface glycoprotein CD3 delta chain; OKT3, delta chain; CD3 antigen, delta subunit; T-cell receptor T3 delta chain; CD3d antigen, delta polypeptide (TiT3 complex)
• UniProt Protein Name: T-cell surface glycoprotein CD3 delta chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD3D
• UniProt Synonym Gene Names: T3D
• UniProt Entry Name: CD3D_HUMAN

NCBI Description

The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]

Uniprot Description

CD3D: a T cell surface glycoprotein that is a component of the T cell antigen receptor. A defect in this protein causes a severe combined immunodeficiency characterized by the absence of T cells but normal B cells. Contains 1 ITAM domain.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; integral to membrane; alpha-beta T cell receptor complex

Molecular Function: transmembrane receptor activity; protein heterodimerization activity; transcription coactivator activity

Biological Process: regulation of immune response; cell surface receptor linked signal transduction; T cell costimulation; positive regulation of transcription from RNA polymerase II promoter; T cell receptor signaling pathway; T cell differentiation; positive thymic T cell selection

Disease: Immunodeficiency 19