Rabbit anti-Rat RAB7A Polyclonal Antibody | anti-RAB7A antibody

Anti-RAB7A Antibody, Rabbit Polyclonal

Cat. Num. AAA8113063

• Gene Names: Rab7a; Rab7
• Reactivity: Rat
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: RAB7A; Polyclonal Antibody; Anti-RAB7A Antibody; Rabbit Polyclonal; RAB7A/Rab-7a Antibody; Rabbit PAb; Antigen Affinity Purified; member RAS oncogene family; Anti-Rab7 Antibody; anti-RAB7A antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rat RAB7A/Rab-7a
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-RAB7A antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:250-1:1000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Rat RAB7A/Rab-7a Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Rat RAB7A/Rab-7a (rR RAB7A/Rab-7a). RAB7A/Rab-7a specific IgG was purified by Rat RAB7A/Rab-7a affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of rat Rab7a in rat skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of rat Rab7a in rat kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunoprecipitation (IP)

(rat Rab7a was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate2 uL anti-rat Rab7a rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-rat Rab7a rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 23 kDa)

Western Blot (WB)

(Anti-RAB7A rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:23 kDaObserved band size:23 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-RAB7A antibody

RAB7A is a ubiquitous small GTPase, which controls transport to late endocytic compartments. Silencing or overexpression of wild type RAB7A changed the soluble/insoluble rate of peripherin indicating that RAB7A is important for peripherin organization and function. Besides, disease-causing RAB7A mutant proteins bind more strongly to peripherin and their expression causes a significant increase in the amount of soluble peripherin. The altered interaction between disease-causing RAB7A mutants and peripherin could play an important role in CMT2B neuropathy.

Product Categories/Family for anti-RAB7A antibody

Autophagy Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 13027392
• NCBI GeneID: 29448
• NCBI Accession #: NP_076440.1
• NCBI GenBank Nucleotide #: NM_023950.3
• UniProt Accession #: P09527; Q4AEF6
• Molecular Weight: 23,504 Da
• NCBI Official Full Name: ras-related protein Rab-7a
• NCBI Official Synonym Full Names: RAB7A, member RAS oncogene family
• NCBI Official Symbol: Rab7a
• NCBI Official Synonym Symbols: Rab7
• NCBI Protein Information: ras-related protein Rab-7a
• UniProt Protein Name: Ras-related protein Rab-7a
• Protein Family: Ras-related protein
• UniProt Gene Name: Rab7a
• UniProt Synonym Gene Names: Rab7

NCBI Description

plays a role in osteoclast polarization and bone resorption during skeletal growth and remodeling [RGD, Feb 2006]

Uniprot Description

Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades (). Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism (). Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes (). Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and Mycobacteria (). Plays a role in the fusion of phagosomes with lysosomes (). Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses (). Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium) (). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts (PubMed:16040606). Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA (PubMed:16306406). Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation (). Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway. Required for the exosomal release of SDCBP, CD63 and syndecan ().