Immunoprecipitation (IP)
(Mouse THOP1 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate2 uL anti-Mouse THOP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse THOP1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 80 kDaObserved band size: 80 kDa)
Rabbit anti-Mouse Thimet Oligopeptidase/THOP1 Polyclonal Antibody | anti-THOP1 antibody
Anti-Thimet Oligopeptidase/THOP1 Antibody, Rabbit Polyclonal
Gene Names
Thop1; EP24.15; AI131655; AI327041
Reactivity
Mouse
Applications
Western Blot, ELISA, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
Thimet Oligopeptidase/THOP1; Polyclonal Antibody; Anti-Thimet Oligopeptidase/THOP1 Antibody; Rabbit Polyclonal; THOP1 Antibody; Rabbit PAb; Antigen Affinity Purified; thimet oligopeptidase 1; Anti-AI131655 Antibody; Anti-AI327041 Antibody; Anti-EP24.15 Antibody; anti-THOP1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse Thimet Oligopeptidase/THOP1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-THOP1 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IP: 4-6uL/mg of lysate
ELISA: 1:5000-1:10,000
IP: 4-6uL/mg of lysate
Immunogen
Recombinant Mouse THOP1 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse THOP1 (rM THOP1). THOP1 specific IgG was purified by Mouse THOP1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunoprecipitation (IP)
(Mouse THOP1 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate2 uL anti-Mouse THOP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse THOP1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 80 kDaObserved band size: 80 kDa)
Immunoprecipitation (IP)
(Mouse THOP1 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate2 uL anti-Mouse THOP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse THOP1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 80 kDaObserved band size: 80 kDa)
Western Blot (WB)
(Anti-THOP1 rabbit polyclonal antibody at 1:500 dilutionLane A: HCT116 Whole Cell LysateLane B: HELA Whole Cell LysateLane C: K562 Whole Cell LysateLane D: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:79 kDaObserved band size:72 kDa)
Western Blot (WB)
(Anti-THOP1 rabbit polyclonal antibody at 1:500 dilutionLane A: HCT116 Whole Cell LysateLane B: HELA Whole Cell LysateLane C: K562 Whole Cell LysateLane D: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:79 kDaObserved band size:72 kDa)
Related Product Information for anti-THOP1 antibody
THOP1, also known as Thimet oligopeptidase 1, Thimet oligopeptidase, EC 3.4.24.15, or EP24.15, is a zinc(II) endopeptidase implicated in the processing of numerous physiological peptides. As an intracellular enzyme, highly expressed in the brain, kidneys and neuroendocrine tissue, THOP1 has been proposed to metabolize peptides within cells, thereby affecting antigen presentation and G protein-coupled receptor signal transduction. Its substrates is gonadotrophin-releasing hormone (GnRH), an important hypothalamic hormone that regulates the synthesis and release of oestradiol and facilitates female sexual behaviour. THOP1 against toxic effects of Abeta in the early stages of Alzheimer disease (AD) pathology, and suggest that the observed increase in THOP1 expression might be part of a compensatory defense mechanism of the brain against an increased Abeta load.
References
Cyr NE, et al. (2010) Nuclear Thimet oligopeptidase is coexpressed with oestrogen receptor alpha in hypothalamic cells and regulated by oestradiol in female mice. J Neuroendocrinol. 22(8): 936-43.Berti DA, et al. (2009) Analysis of intracellular substrates and products of thimet oligopeptidase in human embryonic kidney 293 cells. J Biol Chem. 284(21): 14105-16.Russo LC, et al. (2009) Interaction with calmodulin is important for the secretion of thimet oligopeptidase following stimulation. FEBS J. 276(16): 4358-71.Pollio G, et al. (2008) Increased expression of the oligopeptidase THOP1 is a neuroprotective response to Abeta toxicity. Neurobiol Dis. 31(1): 145-58.Bruce LA, et al. (2008) Hydrogen bond residue positioning in the 599-611 loop of thimet oligopeptidase is required for substrate selection. FEBS J. 275(22): 5607-17.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
78,026 Da
NCBI Official Full Name
thimet oligopeptidase
NCBI Official Synonym Full Names
thimet oligopeptidase 1
NCBI Official Symbol
Thop1
NCBI Official Synonym Symbols
EP24.15; AI131655; AI327041
NCBI Protein Information
thimet oligopeptidase; endopeptidase 24.15
UniProt Protein Name
Thimet oligopeptidase
Protein Family
UniProt Gene Name
Thop1
UniProt Entry Name
THOP1_MOUSE
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Product Notes
The THOP1 thop1 (Catalog #AAA8112119) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Thimet Oligopeptidase/THOP1 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Thimet Oligopeptidase/THOP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 4-6uL/mg of lysate. Researchers should empirically determine the suitability of the THOP1 thop1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thimet Oligopeptidase/THOP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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