Rabbit anti-Mouse Acetylcholinesterase Polyclonal Antibody | anti-ACHE antibody

Anti-Acetylcholinesterase Antibody, Rabbit Polyclonal

Cat. Num. AAA8111728

• Gene Names: Ache; mE1a; mE1b; mE1c; mE1d; mE1e; mE1d'; mE1c-long
• Reactivity: Mouse
• Applications: ELISA
• Purity: Protein A
• Synonyms: Acetylcholinesterase; Polyclonal Antibody; Anti-Acetylcholinesterase Antibody; Rabbit Polyclonal; Acetylcholinesterase/ACHE Antibody; Rabbit PAb; acetylcholinesterase (Yt blood group); Anti-Acre Antibody; Anti-Chrne Antibody; anti-ACHE antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse Acetylcholinesterase/ACHE
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ACHE antibody: ELISA (EIA)
• Application Notes: ELISA: 1:1000-1:2000
• Immunogen: Recombinant Mouse Acetylcholinesterase/ACHE protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse Acetylcholinesterase/ACHE (rM Acetylcholinesterase/ACHE). Total IgG was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-ACHE antibody

Acetylcholinesterase, also known as ACHE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. Acetylcholinesterase plays a crucial role in nerve impulse transmission at cholinergic synapses by rapid hydrolysis of the neurotransmitter acetylcholine (ACh). ACHE appears to be a potential therapeutic target at muscle injuries including organophosphate myopathy. It is an externally oriented membrane-bound enzyme and its main physiological role is termination of chemical transmission at cholinergic synapses and secretory organs by rapid hydrolysis of the neurotransmitter acetylcholine (ACh). ACHE plays important roles in the cholinergic system, and its dysregulation is involved in a variety of human diseases. ACHE was significantly down-regulated in the cancerous tissues of 69.2% of hepatocellular carcinoma (HCC) patients, and the low ACHE expression in HCC was correlated with tumor aggressiveness, an elevated risk of postoperative recurrence, and a low survival rate. Both the recombinant ACHE protein and the enhanced expression of ACHE significantly inhibited HCC cell growth in vitro and tumorigenicity in vivo. ACHE as a tumor growth suppressor in regulating cell proliferation, the relevant signaling pathways, and the drug sensitivity of HCC cells. Thus, ACHE is a promising independent prognostic predictor for HCC recurrence and the survival of HCC patients. ACHE is responsible for the hydrolysis of acetylcholine in the nervous system. It is inhibited by organophosphate and carbamate pesticides. However, this enzyme is only slightly inhibited by organophosphorothionates.

Product Categories/Family for anti-ACHE antibody

Neurotransmitter Associated Enzymes Phosphatases and Regulators

References

Zhao Y, et al. (2011) Acetylcholinesterase, a key prognostic predictor for hepatocellular carcinoma, suppresses cell growth and induces chemosensitization. Hepatology. 53(2): 493-503.Roepcke CB, et al. (2010) Analysis of phosphorothionate pesticides using a chloroperoxidase pretreatment and acetylcholinesterase biosensor detection. J Agric Food Chem. 58(15): 8748-56.Zaheer-ul-Haq, et al. (2010) Benchmarking docking and scoring protocol for the identification of potential acetylcholinesterase inhibitors. J Mol Graph Model. 28(8): 870-82.Pegan K, et al. (2010) Acetylcholinesterase is involved in apoptosis in the precursors of human muscle regeneration. Chem Biol Interact. 187(1-3): 96-100.

NCBI and Uniprot Product Information

• NCBI GI #: 13928664
• NCBI GeneID: 11423
• NCBI Accession #: NP_033729.1
• NCBI GenBank Nucleotide #: NM_009599.3
• UniProt Accession #: P21836
• Molecular Weight: 68,169 Da
• NCBI Official Full Name: acetylcholinesterase
• NCBI Official Synonym Full Names: acetylcholinesterase
• NCBI Official Symbol: Ache
• NCBI Official Synonym Symbols: mE1a; mE1b; mE1c; mE1d; mE1e; mE1d'; mE1c-long
• NCBI Protein Information: acetylcholinesterase
• UniProt Protein Name: Acetylcholinesterase
• Protein Family: Acetylcholinesterase
• UniProt Gene Name: Ache
• UniProt Synonym Gene Names: AChE

Uniprot Description

AChE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.7; Hydrolase; Lipid Metabolism - glycerophospholipid; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 5 G2|5 76.32 cM

Cellular Component: axon; basal lamina; cell junction; cell soma; cell surface; cytoplasm; dendrite; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi apparatus; membrane; neuromuscular junction; neuron projection; nuclear envelope; perinuclear region of cytoplasm; plasma membrane; postsynaptic membrane; presynaptic membrane; rough endoplasmic reticulum; synapse

Molecular Function: acetylcholine binding; acetylcholinesterase activity; choline binding; cholinesterase activity; collagen binding; hydrolase activity; laminin binding; protein binding; protein homodimerization activity; protein self-association; serine hydrolase activity

Biological Process: acetylcholine catabolic process; acetylcholine metabolic process; cell adhesion; choline metabolic process; neurotransmitter catabolic process; neurotransmitter receptor biosynthetic process; positive regulation of axonogenesis; positive regulation of dendrite morphogenesis; protein tetramerization; receptor internalization; regulation of receptor recycling; retina development in camera-type eye; synaptogenesis