Western Blot (WB)
(Anti-CFD rabbit monoclonal antibody at 1:500 dilutionLane A: THP-1 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa)
Rabbit anti-Mouse Factor D Monoclonal Antibody | anti-CFD antibody
Recombinant Anti-Factor D Antibody, Rabbit Monoclonal
Gene Names
Cfd; DF; Adn
Reactivity
Mouse
Applications
Western Blot
Purity
Protein A
Synonyms
Factor D; Monoclonal Antibody; Recombinant Anti-Factor D Antibody; Rabbit Monoclonal; Adipsin/Complement Factor D/CFD Antibody; Rabbit MAb; complement factor D (adipsin); Anti-Adn Antibody; Anti-DF Antibody; anti-CFD antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
104
Specificity
Mouse Adipsin/Complement Factor D/CFD
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CFD antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse Adipsin/Complement Factor D/CFD protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Adipsin/Complement Factor D/CFD (rM Adipsin/Complement Factor D/CFD).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-CFD rabbit monoclonal antibody at 1:500 dilutionLane A: THP-1 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa)
Western Blot (WB)
(Anti-CFD rabbit monoclonal antibody at 1:500 dilutionLane A: THP-1 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa)
Related Product Information for anti-CFD antibody
Complement factor D, also known as Adipsin, C3 convertase activator, Properdin factor D and CFD is a secreted protein which belongs to thepeptidase S1 family. CFD/Adipsin contains onepeptidase S1 domain. Complement factor D (CFD/Adipsin) is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. Complement factor D (CFD/Adipsin) has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Complement factor D (CFD/Adipsin) cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Complement factor D (CFD/Adipsin) is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Defects in CFD/Adipsin are the cause of complement factor D deficiency (CFD deficiency) which predisposes to invasive meningococcal disease.
Product Categories/Family for anti-CFD antibody
References
Volanakis JE, et al., 1996, Protein Sci. 5 (4): 553-64. Searfoss, G.H et al., 2003, J Biol Chem. 278 (46):46107-16. Ukkola, O. et al., 2003, Eur J Clin Nutr. 57 (9):1073-8. Ronti T, et al., 2006, Clin. Endocrinol. (Oxf) 64 (4): 355-65.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,986 Da
NCBI Official Full Name
complement factor D isoform 2
NCBI Official Synonym Full Names
complement factor D (adipsin)
NCBI Official Symbol
Cfd
NCBI Official Synonym Symbols
DF; Adn
NCBI Protein Information
complement factor D
UniProt Protein Name
Complement factor D
Protein Family
UniProt Gene Name
Cfd
UniProt Synonym Gene Names
Adn; Df
UniProt Entry Name
CFAD_MOUSE
Uniprot Description
CFD: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Defects in CFD are the cause of complement factor D deficiency (CFDD). CFDD is an immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Belongs to the peptidase S1 family.
Protein type: Protease; Secreted, signal peptide; EC 3.4.21.46; Secreted
Cellular Component: extracellular region; extracellular space
Molecular Function: endopeptidase activity; hydrolase activity; peptidase activity; serine-type endopeptidase activity; serine-type peptidase activity
Biological Process: complement activation, alternative pathway; immune system process; innate immune response; Notch signaling pathway; proteolysis
Research Articles on CFD
Similar Products
Product Notes
The CFD cfd (Catalog #AAA8111717) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Factor D Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Factor D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the CFD cfd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Factor D, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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