Rabbit anti-Mouse kynureninase/KYNU Polyclonal Antibody | anti-KYNU antibody

Anti-kynureninase/KYNU Antibody, Rabbit Polyclonal

Cat. Num. AAA8111361

• Gene Names: Kynu; 4432411A05Rik
• Reactivity: Mouse
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: kynureninase/KYNU; Polyclonal Antibody; Anti-kynureninase/KYNU Antibody; Rabbit Polyclonal; KYNU Antibody; Rabbit PAb; Antigen Affinity Purified; kynureninase; Anti-4432411A05Rik Antibody; anti-KYNU antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse kynureninase/KYNU
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-KYNU antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Mouse KYNU Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse KYNU (rM KYNU). KYNU specific IgG was purified by Mouse KYNU affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(Mouse KYNU was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate2 uL anti-Mouse KYNU rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse KYNU rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 52 kDaObserved band size: 52 kDa)

Western Blot (WB)

(Anti-KYNU rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:38 kDa)

Related Product Information for anti-KYNU antibody

KYNU (Kynureninase) is a Protein Coding gene. KYNU is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Genetic studies in the mouse and the human suggest that kynureninase activity may influence blood pressure and renal function. The gene coding kynureninase (KYNU) is also located on chromosome band 2q14-q23, where a linkage peak for essential hypertension was previously detected in the Chinese Han population. The results show that the rare KYNU variant Arg188Gln affects kynureninase activity and are consistent with the hypothesis that this mutation can predispose to essential hypertension. Diseases associated with KYNU include Hydroxykynureninuria and Vertebral, Cardiac, Renal, And Limb Defects Syndrome 2.

References

Lima S, et al. (2007) Crystal structure of Homo sapiens kynureninase. Biochemistry. 46(10): 2735-44.Momany C, et al. (2004) Three-dimensional structure of kynureninase from Pseudomonas fluorescens. Biochemistry. 43(5): 1193-203.Toma S, et al. (1997) Cloning and recombinant expression of rat and human kynureninase. FEBS Lett. 408(1): 5-10.

NCBI and Uniprot Product Information

• NCBI GI #: 575771899
• NCBI GeneID: 70789
• NCBI Accession #: NP_001276522.1
• NCBI GenBank Nucleotide #: NM_001289593.1
• UniProt Accession #: Q9CXF0
• Molecular Weight: 52,325 Da
• NCBI Official Full Name: kynureninase isoform 2
• NCBI Official Synonym Full Names: kynureninase (L-kynurenine hydrolase)
• NCBI Official Symbol: Kynu
• NCBI Official Synonym Symbols: 4432411A05Rik
• NCBI Protein Information: kynureninase
• UniProt Protein Name: Kynureninase
• UniProt Gene Name: Kynu
• UniProt Entry Name: KYNU_MOUSE

Uniprot Description

KYNU: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3- hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3- hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity. Xanthurenic aciduria manifesting as massive urinary excretion of large amounts of kynurenine, 3-hydroxykynurenine and xanthurenic acid has been observed in an individual carrying a homozygous missense change in KYNU (PubMed:17334708). The urinary pattern in the patient suggests kynureninase deficiency and a block in the conversion of kynurenine and 3-hydroxykynurenine to anthranilate and 3-hydroxyanthranilate, respectively. Belongs to the kynureninase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - tryptophan; Hydrolase; Mitochondrial; EC 3.7.1.3

Cellular Component: cytoplasm; cytosol; mitochondrion; nucleoplasm

Molecular Function: catalytic activity; hydrolase activity; kynureninase activity; protein homodimerization activity; pyridoxal phosphate binding

Biological Process: anthranilate metabolic process; NAD biosynthetic process; pyridine nucleotide biosynthetic process; quinolinate biosynthetic process; tryptophan catabolic process; tryptophan catabolic process to acetyl-CoA; tryptophan catabolic process to kynurenine