Western Blot (WB)
(Anti-LZTFL1 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:35 kDa (We are unsure as to the identity of these extra bands.))
Rabbit anti-Human LZTFL1 Polyclonal Antibody | anti-LZTFL1 antibody
Anti-LZTFL1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
LZTFL1; Polyclonal Antibody; Anti-LZTFL1 Antibody; Rabbit Polyclonal; LZTFL1 Antibody; Rabbit PAb; Antigen Affinity Purified; anti-LZTFL1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human LZTFL1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-LZTFL1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:200-1:1000
IP: 1-5uL/mg of lysate
IHC-P: 1:200-1:1000
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human LZTFL1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human LZTFL1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-LZTFL1 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:35 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-LZTFL1 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:35 kDa (We are unsure as to the identity of these extra bands.))
Immunohistochemistry (IHC)
(Immunochemical staining of human LZTFL1 in human testis with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human LZTFL1 in human testis with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunoprecipitation (IP)
(LZTFL1 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-LZTFL1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-LZTFL1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size :34 kDa)
Immunoprecipitation (IP)
(LZTFL1 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-LZTFL1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-LZTFL1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size :34 kDa)
Related Product Information for anti-LZTFL1 antibody
Leucine Zipper Transcription Factor-like 1 (LZTFL1) is located in the chromosome region 3p21.3 where allelic loss and genetic alterations occur early and frequently in lung cancers. LZTFL1 is downregulated in epithelial tumors, including lung cancer, and functions as a tumor suppressor in gastric cancers. LZTFL1 inhibits lung tumorigenesis, possibly by maintaining epithelial cell differentiation and/or inhibition of signalings that lead to EMT and suggest that reactivation of LZTFL1 expression in tumor cells may be a novel lung cancer therapeutic approach. LZTFL1 has been identified as a BBS protein (BBS17), because patients with mutations in this gene exhibit the common Bardet-Biedl syndrome (BBS) phenotypes. LZTFL1 is a cilium protein and regulates animal weight and photoreceptor connecting cilium function probably by controlling microtubule assembly and protein trafficking in cilia.
References
Wei Q, et al. (2016) Lztfl1 suppresses lung tumorigenesis by maintaining differentiation of lung epithelial cells. Oncogene 35 (20): 2655-2663.Li S, et al. (2019) Tumor suppressive functions of lztfl1 in hepatocellular carcinoma. Onco Targets Ther 12 5537-5544.Jiang J, et al. (2016) Depletion of bbs protein lztfl1 affects growth and causes retinal degeneration in mice. J Genet Genomics 43 (6): 381-391.
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Product Notes
The LZTFL1 (Catalog #AAA8108662) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LZTFL1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LZTFL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:200-1:1000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the LZTFL1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LZTFL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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