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Immunohistochemistry (IHC) (Immunochemical staining of human CPVL in human lung with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Rabbit anti-Human CPVL Polyclonal Antibody | anti-CPVL antibody

Anti-CPVL Antibody, Rabbit Polyclonal

Gene Names
CPVL; HVLP
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
CPVL; Polyclonal Antibody; Anti-CPVL Antibody; Rabbit Polyclonal; CPVL Antibody; Rabbit PAb; Antigen Affinity Purified; carboxypeptidase; vitellogenic-like; Anti-HVLP Antibody; anti-CPVL antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CPVL
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CPVL antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IHC-P: 1:2500-1:10,000
IP: 1-4uL/mg of lysate
Immunogen
Recombinant Human CPVL protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human CPVL (rh CPVL). CPVL specific IgG was purified by Human CPVL affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human CPVL in human lung with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC) (Immunochemical staining of human CPVL in human lung with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human CPVL in human liver with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC) (Immunochemical staining of human CPVL in human liver with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunoprecipitation (IP)

(CPVL was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-CPVL rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CPVL rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 54 kDaObserved band size: 54 kDa)

Immunoprecipitation (IP) (CPVL was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-CPVL rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CPVL rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 54 kDaObserved band size: 54 kDa)

Western Blot (WB)

(Anti-CPVL rabbit polyclonal antibody at 1:500 dilutionLane A: 293 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:54 kDaObserved band size:54 kDa(We are unsure as to the identity of these extra bands.))

Western Blot (WB) (Anti-CPVL rabbit polyclonal antibody at 1:500 dilutionLane A: 293 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:54 kDaObserved band size:54 kDa(We are unsure as to the identity of these extra bands.))

Western Blot (WB)

(Anti-CPVL rabbit polyclonal antibody at 1:500 dilution Lane A: CPVL konckout 293FT Whole Cell Lysate Lane B: 293FT Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:54 kDa (We are unsure as to the identity of these extra bands.))

Western Blot (WB) (Anti-CPVL rabbit polyclonal antibody at 1:500 dilution Lane A: CPVL konckout 293FT Whole Cell Lysate Lane B: 293FT Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:54 kDa (We are unsure as to the identity of these extra bands.))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
54,164 Da
NCBI Official Full Name
Carboxypeptidase, vitellogenic-like
NCBI Official Synonym Full Names
carboxypeptidase, vitellogenic-like
NCBI Official Symbol
CPVL
NCBI Official Synonym Symbols
HVLP
NCBI Protein Information
probable serine carboxypeptidase CPVL; VCP-like protein; carboxypeptidase WUG; CP-Mac carboxypeptidase; vitellogenic carboxypeptidase-like protein

NCBI Description

The protein encoded by this gene is a carboxypeptidase and bears strong sequence similarity to serine carboxypeptidases. Carboxypeptidases are a large class of proteases that act to cleave a single amino acid from the carboxy termini of proteins or peptides. The exact function of this protein, however, has not been determined. At least two alternatively spliced transcripts which encode the same protein have been observed. [provided by RefSeq, Jul 2008]

Research Articles on CPVL

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Product Notes

The CPVL (Catalog #AAA8107616) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CPVL Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CPVL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IHC-P: 1:2500-1:10,000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the CPVL for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CPVL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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