Rabbit anti-E Coli ssPA Polyclonal Antibody | anti-ssPA antibody

Anti-ssPA Antibody, Rabbit Polyclonal

Cat. Num. AAA8106720

• Gene Names: sspA; ECK3218; JW3198; pog; ssp
• Reactivity: E Coli
• Applications: ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: ssPA; Polyclonal Antibody; Anti-ssPA Antibody; Rabbit Polyclonal; Escherichia coli ssPA/Stringent starvation protein A Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-pog Antibody; Anti-ssp Antibody; anti-ssPA antibody

• Host: Rabbit
• Reactivity: E Coli
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Escherichia coli ssPA/Stringent starvation protein A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ssPA antibody: ELISA (EIA)
• Application Notes: ELISA: 1:5000-1:10,000; Antibody's applications have not been validated with corresponding E Coli.
• Immunogen: Recombinant Escherichia coli ssPA/Stringent starvation protein A protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Escherichia coli Escherichia coli ssPA/Stringent starvation protein A. Escherichia coli ssPA/Stringent starvation protein A specific IgG was purified by Escherichia coli Escherichia coli ssPA/Stringent starvation protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-ssPA antibody

Stringent starvation protein A (ssPA), a member of the GST superfamily, is an RNA polymerase-associated transcriptional activator for the lytic development of phage P1 and is essential for stationary phase-induced acid tolerance of E Coli. It indicates that the flexible regions are not critical for SspA function, whereas the surface pocket is important for both transcriptional activation of the phage P1 late promoter and acid resistance of E Coli. SspA is known to be implicated in survival during nutrient starvation and prolonged stationary phase. Recently, SspA was shown to play an important role in the stationary phase-induced stress response including acid tolerance by down-regulating the level of the global regulator H-NS, which negatively regulates multiple stress defense systems.

References

Hansen AM, et al. (2003) Escherichia coli SspA is a transcription activator for bacteriophage P1 late genes. Mol Microbiol. 48(6): 1621-31.Hommais F, et al. (2001) Large-scale monitoring of pleiotropic regulation of gene expression by the prokaryotic nucleoid-associated protein, H-NS. Mol Microbiol. 40(1): 20-36.Atlung T, et al. (1997) H-NS: a modulator of environmentally regulated gene expression. Mol Microbiol. 24(1): 7-17.Williams MD, et al. (1994) Starvation-induced expression of SspA and SspB: the effects of a null mutation in sspA on Escherichia coli protein synthesis and survival during growth and prolonged starvation. Mol Microbiol. 11(6): 1029-43.

NCBI and Uniprot Product Information

• NCBI GI #: 16131119
• NCBI GeneID: 944744
• NCBI Accession #: NP_417696.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0ACA3; P05838; Q2M8Y4
• Molecular Weight: 24,305 Da
• NCBI Official Full Name: stringent starvation protein A, phage P1 late gene activator, RNAP-associated acid-resistance protein, inactive glutathione S-transferase homolog
• NCBI Official Symbol: sspA
• NCBI Official Synonym Symbols: ECK3218; JW3198; pog; ssp
• NCBI Protein Information: stringent starvation protein A, phage P1 late gene activator, RNAP-associated acid-resistance protein, inactive glutathione S-transferase homolog
• UniProt Protein Name: Stringent starvation protein A
• Protein Family: Stringent starvation protein
• UniProt Gene Name: sspA
• UniProt Synonym Gene Names: pog; ssp

NCBI Description

The SspA protein is associated with RNA polymerase and is required for transcriptional activation of the bacteriophage P1 late promoter in vivo and in vitro . [More information is available at EcoCyc: EG10977].

Uniprot Description

Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme. It is synthesized predominantly when cells are exposed to amino acid starvation, at which time it accounts for over 50% of the total protein synthesized. It is involved in the transition from P1 early to P1 late gene expression. Rnk and SspA can functionally replace P.aeruginosa alginate regulatory gene algR2.