Rabbit anti-Human RhoA Monoclonal Antibody | anti-RHOA antibody

Recombinant Anti-RhoA Antibody (FITC), Rabbit Monoclonal

Cat. Num. AAA8106440

• Gene Names: RHOA; ARHA; ARH12; RHO12; RHOH12
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: RhoA; Monoclonal Antibody; Recombinant Anti-RhoA Antibody (FITC); Rabbit Monoclonal; RhoA Antibody (FITC); Rabbit MAb; ras homolog family member A; Anti-ARH12 Antibody; Anti-ARHA Antibody; Anti-RHO12 Antibody; Anti-RHOH12 Antibody; anti-RHOA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 036
• Specificity: Human RhoA
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 10ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-RHOA antibody: Flow Cytometry (FC/FACS/FCM)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human RhoA protein
• Conjugation: FITC
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human RhoA (rh RhoA) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human RHOA expression in HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), and then stained with FITC Rabbit anti-Human RHOA. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-RHOA antibody

Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein that belongs to the small GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA/ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA/ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila)). RHOA/ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA/ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA/ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP.

Product Categories/Family for anti-RHOA antibody

EGFR Signaling Pathway

References

Kiss C, et al., 1997, Cytogenet. Cell Genet. 79 (3-4): 228-30. Anastasiadis, P.Z. et al., 2000, Nat Cell Biol. 2 (9):637-44. Yiu G, et al., 2006, Nat. Rev. Neurosci. 7 (8): 617-27. Wang, HR. et al., 2006, Methods Enzymol. 406 :437-47. Heo, J. et al., 2006, Biochemistry. 45 (48):14481-9.

NCBI and Uniprot Product Information

• NCBI GI #: 47606458
• NCBI GeneID: 387
• UniProt Accession #: P61586; P06749; Q53HM4; Q5U024; Q9UDJ0; Q9UEJ4
• Molecular Weight: 193
• NCBI Official Full Name: Transforming protein RhoA
• NCBI Official Synonym Full Names: ras homolog family member A
• NCBI Official Symbol: RHOA
• NCBI Official Synonym Symbols: ARHA; ARH12; RHO12; RHOH12
• NCBI Protein Information: transforming protein RhoA; h12; oncogene RHO H12; rho cDNA clone 12; Aplysia ras-related homolog 12; small GTP binding protein RhoA; ras homolog gene family, member A
• UniProt Protein Name: Transforming protein RhoA
• Protein Family: GTP-binding protein
• UniProt Gene Name: RHOA
• UniProt Synonym Gene Names: ARH12; ARHA; RHO12; h12
• UniProt Entry Name: RHOA_HUMAN

Uniprot Description

RHOA: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.

Protein type: G protein, monomeric, Rho; G protein, monomeric; Motility/polarity/chemotaxis; Oncoprotein; G protein

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: apical junction complex; focal adhesion; cytoskeleton; lamellipodium; plasma membrane; cell cortex; midbody; cytosol; cell junction; cleavage furrow

Molecular Function: GTPase activity; protein binding; myosin binding; GTP binding

Biological Process: axon guidance; nerve growth factor receptor signaling pathway; viral reproduction; metabolic process; negative chemotaxis; positive regulation of NF-kappaB import into nucleus; regulation of axonogenesis; regulation of cell migration; Rho protein signal transduction; regulation of osteoblast proliferation; substantia nigra development; transforming growth factor beta receptor signaling pathway; small GTPase mediated signal transduction; positive regulation of stress fiber formation; ephrin receptor signaling pathway; negative regulation of axonogenesis; positive regulation of cytokinesis; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; phosphoinositide-mediated signaling; apical junction assembly; regulation of small GTPase mediated signal transduction; positive regulation of axonogenesis; actin cytoskeleton organization and biogenesis; positive regulation of neuron differentiation; vascular endothelial growth factor receptor signaling pathway; blood coagulation