Rabbit anti-Human ATP citrate lyase/ACLY Polyclonal Antibody | anti-ACLY antibody

Anti-ATP citrate lyase/ACLY Antibody, Rabbit Polyclonal

Cat. Num. AAA8105797

• Gene Names: ACLY; ACL; ATPCL; CLATP
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: ATP citrate lyase/ACLY; Polyclonal Antibody; Anti-ATP citrate lyase/ACLY Antibody; Rabbit Polyclonal; ACLY/ATP Antibody; Rabbit PAb; Antigen Affinity Purified; ATP citrate lyase; Anti-ACL Antibody; Anti-ATPCL Antibody; Anti-CLATP Antibody; anti-ACLY antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ATP citrate lyase/ACLY
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ACLY antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human ACLY/ATP Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human ACLY/ATP (rh ACLY/ATP). ACLY/ATP specific IgG was purified by Human ACLY/ATP affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(ACLY was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-ACLY rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ACLY rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 120 kDaObserved band size: 120 kDa)

Western Blot (WB)

(Anti-ACLY rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:120 kDaObserved band size:120 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-ACLY antibody

ATP citrate lyase, also known as Acly or Acl, is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is composed of two polymer chains which are polypeptides in human. ATP citrate lyase is responsible for catalyzing the conversion of citrate and CoA into acetyl-CoA and oxaloacetate, along with the hydrolysis of ATP. A definitive role for ATP citrate lyase in tumorigenesis has emerged from ATP citrate lyase RNAi and chemical inhibitor studies, showing that ATP citrate lyase inhibition limits tumor cell proliferation and survival and induces differentiation in vitro. In vivo, it reduces tumor growth leading to a cytostatic effect and induces differentiation.

References

Kim W, et al. (2006) Both subunits of ATP-citrate lyase from Chlorobium tepidum contribute to catalytic activity. J Bacteriol. 188 (18) : 6544-52.Ki SW, et al. (2000) Radicicol binds and inhibits mammalian ATP citrate lyase. J Biol Chem. 275 (50) : 39231-6.Schneider K, et al. (2000) Biosynthesis of the prosthetic group of citrate lyase. Biochemistry. 39 (31) : 9438-50.

NCBI and Uniprot Product Information

• NCBI GI #: 38569421
• NCBI GeneID: 47
• NCBI Accession #: NP_001087.2
• NCBI GenBank Nucleotide #: NM_001096.2
• UniProt Accession #: P53396; Q13037; Q9BRL0; B4DIM0
• Molecular Weight: 120,839 Da
• NCBI Official Full Name: ATP-citrate synthase isoform 1
• NCBI Official Synonym Full Names: ATP citrate lyase
• NCBI Official Symbol: ACLY
• NCBI Official Synonym Symbols: ACL; ATPCL; CLATP
• NCBI Protein Information: ATP-citrate synthase; ATP citrate synthase; citrate cleavage enzyme; ATP-citrate (pro-S-)-lyase
• UniProt Protein Name: ATP-citrate synthase
• Protein Family: ATP-citrate synthase
• UniProt Gene Name: ACLY
• UniProt Synonym Gene Names: ACL
• UniProt Entry Name: ACLY_HUMAN

NCBI Description

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ACLY: ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. Homotetramer. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.3.8; Transferase; Carbohydrate Metabolism - citrate (TCA) cycle; Lyase

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: nucleoplasm; membrane; mitochondrion; citrate lyase complex; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; ATP citrate synthase activity; metal ion binding; succinate-CoA ligase (ADP-forming) activity; cofactor binding; ATP binding

Biological Process: coenzyme A metabolic process; lipid biosynthetic process; positive regulation of cellular metabolic process; energy reserve metabolic process; citrate metabolic process; cellular carbohydrate metabolic process; triacylglycerol biosynthetic process; cellular lipid metabolic process