Western Blot (WB)
(Anti-SETD8 rabbit polyclonal antibody at 1:500 dilutionLane A: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:43 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human PR-Set7 Polyclonal Antibody | anti-KMT5A antibody
Anti-PR-Set7 Antibody, Rabbit Polyclonal
ELISA: 1:25,000-1:50,000
Western Blot (WB)
(Anti-SETD8 rabbit polyclonal antibody at 1:500 dilutionLane A: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:43 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-SETD8 rabbit polyclonal antibody at 1:500 dilutionLane A: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:43 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
NCBI and Uniprot Product Information
Uniprot Description
SETD8: a protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Methylates K20 of histone H4 (H4K20me1), a specific tag for epigenetic transcriptional repression. SETD8 protein and H4K20me1 levels are cell cycle regulated, both increasing in S phase and peaking at G2/M phase. Interacts with the PCNA protein, associates with sites of active DNA synthesis and is required for DNA replication and genome stability during S phase. Inhibition of SET8 using shRNA results in arrest of replication forks, induction of double-stranded DNA breaks and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Inhibition of SET8 using shRNA results in arrest of replication forks, induction of double-stranded DNA breaks and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle. Nucleosomes are preferred as substrate compared to free histones. Methylates p53K382, leading to repression of the pro-apoptotic and checkpoint activation functions of p53. SET8 expression levels decrease in response to DNA damage, allowing p53 to activate checkpoints and/or apoptosis. Both the methylation of histone H4K20 and p53K382 appear to be important for the functions of SET8 in S phase. Belongs to the histone-lysine methyltransferase family. PR/SET subfamily. Two isoforms of the human protein are produced by alternative splicing.
Protein type: Methyltransferase, protein lysine; Methyltransferase; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation
Chromosomal Location of Human Ortholog: 12q24.31
Cellular Component: nucleoplasm; nucleolus; chromosome; nucleus
Molecular Function: protein binding; p53 binding; protein-lysine N-methyltransferase activity; transcription corepressor activity; histone-lysine N-methyltransferase activity
Biological Process: mitosis; transcription, DNA-dependent; cell division; peptidyl-lysine mono-methylation; negative regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; regulation of DNA damage response, signal transduction by p53 class mediator
Research Articles on KMT5A
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Product Notes
The KMT5A setd8 (Catalog #AAA8105729) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PR-Set7 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PR-Set7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000. Researchers should empirically determine the suitability of the KMT5A setd8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PR-Set7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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