Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CUEDC2 expression on SHSY5Y cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human CUEDC2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Mouse anti-Human CUEDC2 Monoclonal Antibody | anti-CUEDC2 antibody
Anti-CUEDC2 Antibody, Mouse Monoclonal
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
CUEDC2; Monoclonal Antibody; Anti-CUEDC2 Antibody; Mouse Monoclonal; CUEDC2 Antibody; Mouse MAb; CUE domain containing 2; Anti-bA18I14.5 Antibody; Anti-C10orf66 Antibody; anti-CUEDC2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
08
Specificity
Human CUEDC2
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CUEDC2 antibody
Flow Cytometry (FC/FACS/FCM)
Application Notes
FC/FACS/FCM: 1:25-1:100
Immunogen
Recombinant Human CUEDC2 Protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CUEDC2 (rh CUEDC2; NP_076945.2; aa1-287). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CUEDC2 expression on SHSY5Y cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human CUEDC2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CUEDC2 expression on SHSY5Y cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human CUEDC2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-CUEDC2 antibody
CUE domain-containing 2 (CUEDC2) is a protein involved in the regulation of the cell cycle, inflammation, and tumorigenesis and is highly expressed in many types of tumors. CUEDC2 is phosphorylated by Cdk1 during mitosis and promotes the release of anaphase-promoting complex or cyclosome (APC/C) from checkpoint inhibition. CUEDC2 is also known to interact with IkB kinase alpha (IKKalpha) and IKKbeta and has an inhibitory role in the activation of transcription factor nuclear factor-kappaB. Moreover, CUEDC2 plays an important role in downregulating the expression of hormone receptors estrogen receptor-alpha and progesterone receptor, thereby impairing the responsiveness of breast cancer to endocrine therapies. The expression level of CUEDC2 has an inverse correlation with imatinib resistance and activity of NF-kappaB signaling pathway in CML cells, CUEDC2 could regulate imatinib sensitivity in CML cells at least partially through NF-kappaB signaling pathway. CUEDC2 may be a promising biomarker to evaluate the progression of serous ovarian carcinoma and to predict likely relapse of ovarian serous carcinoma. The decreased expression of CUEDC2 contributes to tumor growth in lung adenocarcinoma, leading to a poor clinical outcome.
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Product Notes
The CUEDC2 (Catalog #AAA8105341) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CUEDC2 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CUEDC2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the CUEDC2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CUEDC2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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