Rabbit anti-Human CEACAM5 Monoclonal Antibody | anti-CEACAM5 antibody

Recombinant Anti-CEACAM5 Antibody (PE), Rabbit Monoclonal

Cat. Num. AAA8104812

• Gene Names: CEACAM5; CEA; CD66e
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: CEACAM5; Monoclonal Antibody; Recombinant Anti-CEACAM5 Antibody (PE); Rabbit Monoclonal; CEACAM5/CD66e Antibody (PE); Rabbit MAb; carcinoembryonic antigen-related cell adhesion molecule 5; Anti-CD66e Antibody; Anti-CEA Antibody; anti-CEACAM5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 061
• Specificity: Human CEACAM5/CD66e
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 2ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-CEACAM5 antibody: Flow Cytometry (FC/FACS/FCM)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human CEACAM5/CD66e protein
• Conjugation: PE
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CEACAM5/CD66e (rh CEACAM5/CD66e) and conjugated with PE under optimum conditions, the unreacted PE was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of anti-Human CEACAM5 on HT29 cells.)

Related Product Information for anti-CEACAM5 antibody

CEACAM5, also known as CEA or D66e, belongs to the large CEACAM subfamily of the immunoglobulin superfamily. CEACAM5 is expressed primarily by epithelial cells, and is synthesized as a glycoprotein with an MW of 180 kDa comprising 60% carbohydrate. CEACAM5 contains one Ig-like V-type domain at the N-terminus, followed by six Ig-like C2-type domains and a GPI anchor, and exists as a homodimer. CEACAM5 and CEACAM6 are overexpressed in many cancers and are associated with adhesion and invasion. CEACAM5 can mediate cell-cell adhesion through homotypic and heterotypic interactions. It functions as a homotypic intercellular adhesion molecule and serves as a widely used tumor marker, since it is expressed at higher levels in tumorous tissues than in corresponding normal tissues. CEACAM5 has also been shown to contribute to tumorigenicity by inhibiting cellular differentiation. In addition, CEACAM5 is identified as the host receptor for the Dr family of adhesins of E Coli, and the binding of E Coli Dr adhesins leads to dissociation of the CEACAM5 homodimer.

Product Categories/Family for anti-CEACAM5 antibody

Cancer Drug Targets

References

Baczyska D, et al. (2003) The tumorigenic potential of human CX-1 colon adenocarcinoma cells depends on carcinoembryonic antigen (CEACAM5) expression. Cell Mol Biol Lett. 8(2): 471-86.
Blumenthal RD, et al. (2005) Inhibition of adhesion, invasion, and metastasis by antibodies targeting CEACAM6 (NCA-90) and CEACAM5 (Carcinoembryonic Antigen). Cancer Res. 65(19): 8809-17.
Liebig B, et al. (2005) Forced expression of deltaN-TCF-1B in colon cancer derived cell lines is accompanied by the induction of CEACAM5/6 and mesothelin. Cancer Lett. 223(1): 159-67.

NCBI and Uniprot Product Information

• NCBI GI #: 98986445
• NCBI GeneID: 1048
• NCBI Accession #: NP_004354.2
• NCBI GenBank Nucleotide #: NM_004363.2
• UniProt Accession #: P06731
• Molecular Weight: 77kDa
• NCBI Official Full Name: carcinoembryonic antigen-related cell adhesion molecule 5 preproprotein
• NCBI Official Synonym Full Names: carcinoembryonic antigen-related cell adhesion molecule 5
• NCBI Official Symbol: CEACAM5
• NCBI Official Synonym Symbols: CEA; CD66e
• NCBI Protein Information: carcinoembryonic antigen-related cell adhesion molecule 5; meconium antigen 100
• UniProt Protein Name: Carcinoembryonic antigen-related cell adhesion molecule 5
• Protein Family: Carcinoembryonic antigen-related cell adhesion molecule
• UniProt Gene Name: CEACAM5
• UniProt Synonym Gene Names: CEA; CEA
• UniProt Entry Name: CEAM5_HUMAN

Uniprot Description

CEACAM5: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Belongs to the immunoglobulin superfamily. CEA family.

Protein type: Membrane protein, integral; Immunoglobulin superfamily; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: basolateral plasma membrane; integral to plasma membrane

Molecular Function: identical protein binding; protein homodimerization activity

Biological Process: homotypic cell-cell adhesion; negative regulation of apoptosis