Mouse anti-Human 14-3-3 epsilon Monoclonal Antibody | anti-YWHAE antibody

Anti-14-3-3 epsilon Antibody, Mouse Monoclonal

Cat. Num. AAA8104148

• Gene Names: YWHAE; MDS; MDCR; KCIP-1; 14-3-3E
• Reactivity: Human
• Applications: ELISA
• Purity: Protein A
• Synonyms: 14-3-3 epsilon; Monoclonal Antibody; Anti-14-3-3 epsilon Antibody; Mouse Monoclonal; 14-3-3 epsilon/YWHAE Antibody; Mouse MAb; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; epsilon; Anti-14-3-3E Antibody; Anti-HEL2 Antibody; Anti-KCIP-1 Antibody; Anti-MDCR Antibody; Anti-MDS Antibody; anti-YWHAE antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 07
• Specificity: Human 14-3-3 epsilon/YWHAE
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-YWHAE antibody: ELISA (EIA)
• Application Notes: ELISA: 1:1000-1:2000
• Immunogen: Recombinant Human 14-3-3 epsilon/YWHAE Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 epsilon/YWHAE (rh 14-3-3 epsilon/YWHAE). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-YWHAE antibody

YWHAE, also known as 14-3-3 epsilon, mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 epsilon/YWHAE is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 epsilon/YWHAE is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. YWHAE interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 epsilon/YWHAE is implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 epsilon/YWHAE Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. This Binding generally results in the modulation of the activity of the binding partner.

Product Categories/Family for anti-YWHAE antibody

phospho-Serine/phospho-Threonine Binding Proteins Adaptor Proteins Apoptosis Adaptor Proteins; Death Receptor Signaling

References

Ikeda M, et al. (2008) Identification of YWHAE, a gene encoding 14-3-3epsilon, as a possible susceptibility gene for schizophrenia. Hum Mol Genet. 17(20): 3212-22.Mignon-Ravix C, et al. (2010) Deletion of YWHAE in a patient with periventricular heterotopias and pronounced corpus callosum hypoplasia. J Med Genet. 47(2): 132-6.Nagamani SC, et al. (2009) Microdeletions including YWHAE in the Miller-Dieker syndrome region on chromosome 17p13.3 result in facial dysmorphisms, growth restriction, and cognitive impairment. J Med Genet. 46(12): 825-33.

NCBI and Uniprot Product Information

• NCBI GI #: 5803225
• NCBI GeneID: 7531
• NCBI Accession #: NP_006752.1
• NCBI GenBank Nucleotide #: NM_006761.4
• UniProt Accession #: P62258; P29360; P42655; Q4VJB6; Q53XZ5; Q63631; Q7M4R4; B3KY71; D3DTH5
• Molecular Weight: 29,174 Da
• NCBI Official Full Name: 14-3-3 protein epsilon
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
• NCBI Official Symbol: YWHAE
• NCBI Official Synonym Symbols: MDS; MDCR; KCIP-1; 14-3-3E
• NCBI Protein Information: 14-3-3 protein epsilon; 14-3-3 epsilon; protein kinase C inhibitor protein-1; mitochondrial import stimulation factor L subunit; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide
• UniProt Protein Name: 14-3-3 protein epsilon
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAE
• UniProt Synonym Gene Names: 14-3-3E
• UniProt Entry Name: 1433E_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

14-3-3 epsilon: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: kinesin complex; cytoplasmic vesicle membrane; focal adhesion; mitochondrion; membrane; axon; melanosome; cytosol

Molecular Function: protein domain specific binding; protein binding; enzyme binding; potassium channel regulator activity; protein heterodimerization activity; histone deacetylase binding; phosphoprotein binding; phosphoserine binding

Biological Process: substantia nigra development; nerve growth factor receptor signaling pathway; viral reproduction; apoptosis; organelle organization and biogenesis; hippocampus development; neuron migration; mitotic cell cycle; cerebral cortex development; regulation of caspase activity; G2/M transition of mitotic cell cycle; regulation of the rate of heart contraction by hormone; protein targeting

Disease: Miller-dieker Lissencephaly Syndrome