Rabbit PSMD5 Polyclonal Antibody | anti-PSMD5 antibody

Anti-PSMD5 Antibody, Rabbit Polyclonal

Cat. Num. AAA8104032

• Gene Names: PSMD5; S5B
• Reactivity: Human; Predicted to React with: Bovine, Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: PSMD5; Polyclonal Antibody; Anti-PSMD5 Antibody; Rabbit Polyclonal; PSMD5 Antibody; Rabbit PAb; Antigen Affinity Purified; proteasome 26S subunit; non-ATPase 5; Anti-S5B Antibody; anti-PSMD5 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Bovine, Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PSMD5
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PSMD5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Human PSMD5
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PSMD5, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PSMD5 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:56 kDa Observed band size:57 kDa)

Related Product Information for anti-PSMD5 antibody

PSMD5 (Proteasome 26S Subunit, Non-ATPase 5) is a Protein Coding gene. The PSMD5 gene, located on 9q33.2, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, and frog. This gene encodes a non-ATPase subunit of the 19S regulator base that functions as a chaperone protein during 26S proteasome assembly. PSMD5 is widely expressed in the urinary bladder, adrenal, and other tissues. Diseases associated with PSMD5 include Localized Pulmonary Fibrosis and Kaufman Oculocerebrofacial Syndrome. The related pathways of PSMD5 are Regulation of activated PAK-2p34 by proteasome-mediated degradation and Antigen processing-Cross presentation. 242 organisms have orthologs with the human gene PSMD5.

References

Kanayama HO, et al. (1992) Demonstration that a human 26s proteolytic complex consists of a proteasome and multiple associated protein components and hydrolyzes atp and ubiquitin-ligated proteins by closely linked mechanisms. Eur J Biochem 206 (2): 567-578.Deveraux Q, et al. (1995) Molecular cloning and expression of a 26 s protease subunit enriched in dileucine repeats. J Biol Chem 270 (40): 23726-23729.Deveraux Q, et al. (1994) A 26 s protease subunit that binds ubiquitin conjugates. J Biol Chem 269 (10): 7059-7061.

NCBI and Uniprot Product Information

• NCBI GI #: 3122657
• NCBI GeneID: 5711
• UniProt Accession #: Q16401; Q15045; Q4VXG8; B4DZM8
• Molecular Weight: 504
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 5
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 5
• NCBI Official Symbol: PSMD5
• NCBI Official Synonym Symbols: S5B
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 5; 26S proteasome subunit S5B; 26S protease subunit S5 basic
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 5
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD5
• UniProt Synonym Gene Names: KIAA0072
• UniProt Entry Name: PSMD5_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a non-ATPase subunit of the 19S regulator base that functions as a chaperone protein during 26S proteasome assembly. [provided by RefSeq, Jul 2012]

Uniprot Description

PSMD5: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD5:PSMC2:PSMC1:PSMD2 module which probably assembles with a PSMD10:PSMC4:PSMC5:PAAF1 module followed by dissociation of PSMD5. Belongs to the proteasome subunit S5B/HSM3 family.

Protein type: Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 9q33.2

Cellular Component: nucleoplasm; proteasome complex; cytosol

Molecular Function: protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis