Rabbit anti-Mouse MYL2 Polyclonal Antibody | anti-MYL2 antibody

Anti-MYL2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103783

• Gene Names: Myl2; MLC-2; Mlc2v; Mylpc; MLC-2v
• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: MYL2; Polyclonal Antibody; Anti-MYL2 Antibody; Rabbit Polyclonal; MYL2 Antibody; Rabbit PAb; Antigen Affinity Purified; anti-MYL2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse MYL2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-MYL2 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse MYL2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse MYL2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MYL2 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse heart tissue lysate Lane B: Mouse muscle tissue lysate Lysates/proteins at 30 ug per Lane. Secondary Goat Anti-Rabbit-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:19 kDa Observed band size:19 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse MYL2 in mouse heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse MYL2 in mouse skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse MYL2 in rat heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse MYL2 in rat skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-MYL2 antibody

Myosin regulatory light chain-2 (MYL2), also called MLC-2, is an ~19kDa sarcomeric protein that belongs to the EF-hand calcium binding protein superfamily and exists as three major isoforms encoded by three distinct genes in mammalian striated muscle. Each of the three different MLC-2 genes (MLC-2f; fast twitch skeletal isoform, MLC-2v; cardiac ventricular and slow twitch skeletal isoform, MLC-2a; cardiac atrial isoform) has a distinct developmental expression pattern in mammals. Genetic loss-of-function studies in mice demonstrated an essential role for cardiac isoforms of MLC-2, MLC-2v and MLC-2a, in cardiac contractile function during early embryogenesis. MYL2 encodes a myosin regulatory light chain (MLC-2V). The myosin regulatory light chains bind, together with the essential light chains, to the flexible neck region of the myosin heavy chain in the hexameric myosin complex and have a structural and regulatory role in muscle contraction. The MYL2 mutations are associated with severe cardiac hypertrophy and sudden cardiac death (SCD).

References

Weterman MA, et al. (2013) Recessive myl2 mutations cause infantile type i muscle fibre disease and cardiomyopathy. Brain 136 (Pt 1): 282-293.Sheikh F, et al. (2015) Functions of myosin light chain-2 (myl2) in cardiac muscle and disease. Gene 569 (1): 14-20.Li Y, et al. (2011) Slow cardiac myosin regulatory light chain 2 (myl2) was down-expressed in chronic heart failure patients. Clin Cardiol 34 (1): 30-34.

NCBI and Uniprot Product Information

• NCBI GI #: 153791853
• NCBI GeneID: 17906
• NCBI Accession #: NP_034991.3
• NCBI GenBank Nucleotide #: NM_010861.3
• UniProt Accession #: P51667; Q6P8P4; Q9QVP3
• Molecular Weight: 18,864 Da
• NCBI Official Full Name: myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• NCBI Official Synonym Full Names: myosin, light polypeptide 2, regulatory, cardiac, slow
• NCBI Official Symbol: Myl2
• NCBI Official Synonym Symbols: MLC-2; Mlc2v; Mylpc; MLC-2v
• NCBI Protein Information: myosin regulatory light chain 2, ventricular/cardiac muscle isoform; myosin light chain 2v; ventricular myosin regulatory light chain; myosin light chain, phosphorylatable, cardiac ventricles
• UniProt Protein Name: Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• Protein Family: Myosin regulatory
• UniProt Gene Name: Myl2
• UniProt Synonym Gene Names: Mylpc; MLC-2; MLC-2v
• UniProt Entry Name: MLRV_MOUSE

Uniprot Description

MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Protein type: Contractile

Cellular Component: cytoskeleton; contractile fiber; myofibril; cytoplasm; myosin complex; actin cytoskeleton

Molecular Function: actin monomer binding; metal ion binding; calcium ion binding

Biological Process: heart morphogenesis; muscle cell fate specification; heart contraction; heart development; ventricular cardiac muscle morphogenesis; cardiac myofibril assembly; negative regulation of cell growth; muscle fiber development; post-embryonic development; cardiac muscle contraction