Mouse anti-Human PBK/TOPK Monoclonal Antibody | anti-PBK antibody

Anti-PBK/TOPK Antibody, Mouse Monoclonal

Cat. Num. AAA8103716

• Gene Names: PBK; SPK; CT84; TOPK; Nori-3
• Reactivity: Human
• Applications: Western Blot, ELISA, ELISA
• Purity: Protein A
• Synonyms: PBK/TOPK; Monoclonal Antibody; Anti-PBK/TOPK Antibody; Mouse Monoclonal; PDK/PDZ binding kinase/TOPK Antibody; Mouse MAb; PDZ binding kinase; Anti-CT84 Antibody; Anti-HEL164 Antibody; Anti-Nori-3 Antibody; Anti-SPK Antibody; Anti-TOPK Antibody; anti-PBK antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 1E5E5E6
• Specificity: Human PBK/TOPK/CT84
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PBK antibody: Western Blot (WB), ELISA (EIA), ELISA (EIA) (Det)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:5000-1:10,000; ELISA (Det): 1:5000-1:50,000; ELISA (Det): This antibody will detect Human PBK/TOPK in ELISA pair set.; In a sandwich ELISA, it can be used as detection antibody when paired with.
• Immunogen: Recombinant Human PBK protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PBK/TOPK (rh PBK). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-PBK antibody

PDZ binding kinase (PBK), also known as TOPK (T-LAK cell-originated protein kinase), is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family, and has all the characteristic protein kinase subdomains and a C-terminal PDZ-binding T/SXV motif. PBK is expressed in the testis restrictedly expressed in outer cell layer of seminiferous tubules, as well as placenta. PBK may be enrolled in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. This mitotic kinase phosphorylates MAP kinase p38 and seems to be active in mitosis. When phosphorylated, PBK forms a protein-protein interaction with tumor suppressor p53 (TP53), leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. The expression level of PBK is thus upregulated in a variety of neoplasms including hematological malignancies.

References

Zakut-Houri R., et al., (1985), Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4:1251-1255.Lamb P., et al., (1986), Characterization of the human p53 gene.Mol. Cell. Biol. 6:1379-1385.Harlow E., et al., (1985), Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.Mol. Cell. Biol. 5:1601-1610.

NCBI and Uniprot Product Information

• NCBI GI #: 18490991
• NCBI GeneID: 55872
• NCBI Accession #: NP_060962.2
• NCBI GenBank Nucleotide #: NM_018492.2
• UniProt Accession #: Q96KB5; Q9NPD9; Q9NYL7; Q9NZK6; D3DST2
• Molecular Weight: 36,085 Da
• NCBI Official Full Name: lymphokine-activated killer T-cell-originated protein kinase
• NCBI Official Synonym Full Names: PDZ binding kinase
• NCBI Official Symbol: PBK
• NCBI Official Synonym Symbols: SPK; CT84; TOPK; Nori-3
• NCBI Protein Information: lymphokine-activated killer T-cell-originated protein kinase; PDZ-binding kinase; cancer/testis antigen 84; MAPKK-like protein kinase; serine/threonine protein kinase; T-LAK cell-originated protein kinase; spermatogenesis-related protein kinase
• UniProt Protein Name: Lymphokine-activated killer T-cell-originated protein kinase
• Protein Family: Lymphokine-activated killer T-cell-originated protein kinase
• UniProt Gene Name: PBK
• UniProt Synonym Gene Names: TOPK; CT84; SPK
• UniProt Entry Name: TOPK_HUMAN

NCBI Description

This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

PBK: Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. Interacts with DLG1 and TP53. Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. Activated by phosphorylation. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Protein type: Protein kinase, Other; Cancer Testis Antigen (CTA); Protein kinase, Ser/Thr (non-receptor); EC 2.7.12.2; Kinase, protein; Other group; TOPK family

Chromosomal Location of Human Ortholog: 8p21.2

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: mitosis; negative regulation of protein amino acid phosphorylation; negative regulation of inflammatory response; negative regulation of stress-activated MAPK cascade; protein amino acid phosphorylation; negative regulation of proteasomal ubiquitin-dependent protein catabolic process