Rabbit NEURL1 Polyclonal Antibody | anti-NEURL1 antibody

Anti-NEURL1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102808

• Gene Names: NEURL; neu; RNF67; neu-1; NEURL1; bA416N2.1
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: NEURL1; Polyclonal Antibody; Anti-NEURL1 Antibody; Rabbit Polyclonal; NEURL1 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-bA416N2.1 Antibody; Anti-neu Antibody; Anti-neu-1 Antibody; Anti-NEURL Antibody; Anti-RNF67 Antibody; anti-NEURL1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NEURL1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-NEURL1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human NEURL1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human NEURL1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NEURL1 rabbit polyclonal antibody at 1:500 dilution Lane A: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:62 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of NEURL1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NEURL1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Related Product Information for anti-NEURL1 antibody

Drosophila Neur (dNeur) protein has two Neuralized Homology Repeat (NHR) domains and a C-terminal C3HC4 RING Zn-finger (RING) domain. In mammals, there are at least four proteins with NHR domains: NEURL1 (or NEURL, NEURL1A, NEUR1) and NEURL1B (or NEUR2) both have two NHR domains and a C-terminal RING domain, whereas NEURL2 (or Ozz-E3) and NEURL3 (or LINCR) have only one NHR domain and a C-terminal SOCS (suppressor of cytokines signalling) or RING domain, respectively. Mammalian NEURL1 and NEURL1B proteins interact with and ubiquitinate Notch ligands Delta-like1, Delta-like4 and Jagged1. Human Neuralized1 (NEURL1) was investigated as a potential tumor suppressor in medulloblastoma (MB). The gene is located at 10q25.1, a region demonstrating frequent loss of heterozygosity in tumors.

References

Taal K, et al. (2019) Neuralized family member neurl1 is a ubiquitin ligase for the cgmp-specific phosphodiesterase 9a. Sci Rep 9 (1): 7104.

NCBI and Uniprot Product Information

• NCBI GI #: 223278368
• NCBI GeneID: 9148
• NCBI Accession #: NP_004201.3
• NCBI GenBank Nucleotide #: NM_004210.4
• UniProt Accession #: O76050; Q5TDR2; Q5TDR3; Q8TAN0; Q9H463
• Molecular Weight: 61,860 Da
• NCBI Official Full Name: neuralized-like protein 1A
• NCBI Official Synonym Full Names: neuralized homolog (Drosophila)
• NCBI Official Symbol: NEURL
• NCBI Official Synonym Symbols: neu; RNF67; neu-1; NEURL1; bA416N2.1
• NCBI Protein Information: neuralized-like protein 1A; h-neuralized 1; RING finger protein 67
• UniProt Protein Name: Neuralized-like protein 1A
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: NEURL
• UniProt Synonym Gene Names: NEURL1; NEURL1A; RNF67; h-neu
• UniProt Entry Name: NEU1A_HUMAN

Uniprot Description

NEURL1: May function as an E3 ubiquitin-protein ligase to activate monoubiquitination of JAG1, thereby regulating the Notch pathway. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Motility/polarity/chemotaxis; Ligase; Ubiquitin conjugating system; EC 6.3.2.-; Cell development/differentiation; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 10q25.1

Cellular Component: postsynaptic membrane; perinuclear region of cytoplasm; postsynaptic density; dendritic spine; plasma membrane; perikaryon; cell junction

Molecular Function: translation factor activity, non-nucleic acid binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: lactation; positive regulation of filopodium formation; protein monoubiquitination; nervous system development; Notch signaling pathway; positive regulation of apoptosis; positive regulation of long-term neuronal synaptic plasticity; regulation of translation; negative regulation of cell proliferation; sperm motility; positive regulation of epidermal growth factor receptor activity; sperm axoneme assembly; negative regulation of Notch signaling pathway