Western Blot (WB)
(Anti-CTAGE1 rabbit polyclonal antibody at 1:500 dilutionLane A: MDA-MB453 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:85 kDaObserved band size:85 kDa)
Rabbit anti-Human CTAGE1 Polyclonal Antibody | anti-CTAGE1 antibody
Anti-CTAGE1 Antibody, Rabbit Polyclonal
Gene Names
CTAGE1; CTAGE; CT21.1; CT21.2; CTAGE-1; CTAGE-2
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
CTAGE1; Polyclonal Antibody; Anti-CTAGE1 Antibody; Rabbit Polyclonal; CTAGE1 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-CT21.2 Antibody; Anti-CTAGE Antibody; Anti-CTAGE-1 Antibody; Anti-CTAGE-2 Antibody; Anti-hCG_2039601 Antibody; anti-CTAGE1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CTAGE1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CTAGE1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IP: 0.2-1uL/mg of lysate
IP: 0.2-1uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human CTAGE1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human CTAGE1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-CTAGE1 rabbit polyclonal antibody at 1:500 dilutionLane A: MDA-MB453 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:85 kDaObserved band size:85 kDa)
Western Blot (WB)
(Anti-CTAGE1 rabbit polyclonal antibody at 1:500 dilutionLane A: MDA-MB453 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:85 kDaObserved band size:85 kDa)
Immunoprecipitation (IP)
(CTAGE1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate0.5 uL anti-CTAGE1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CTAGE1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 85 kDaObserved band size: 85 kDa)
Immunoprecipitation (IP)
(CTAGE1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate0.5 uL anti-CTAGE1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CTAGE1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 85 kDaObserved band size: 85 kDa)
Related Product Information for anti-CTAGE1 antibody
CTAGE1 is a gene on chromosome 18p11.2 that encodes cutaneous T-cell lymphoma-associated antigen 1, which is expressed in about 35% of CTCL tumours, including cutaneous T-cell lymphoma, mycosis fungoides and Sezary syndrome. An important paralog of this gene is MIA2.
References
Akiyama Y, et al. (2014) Novel cancer-testis antigen expression on glioma cell lines derived from high-grade glioma patients. Oncol Rep 31 (4): 1683-1690.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85,282 Da
NCBI Official Full Name
cTAGE family member 2 isoform 1
NCBI Official Synonym Full Names
cutaneous T-cell lymphoma-associated antigen 1
NCBI Official Symbol
CTAGE1
NCBI Official Synonym Symbols
CTAGE; CT21.1; CT21.2; CTAGE-1; CTAGE-2
NCBI Protein Information
cTAGE family member 2; protein cTAGE-2; cancer/testis antigen 21.2; cancer/testis antigen family 21, member 1; cancer/testis antigen family 21, member 2; cutaneous T-cell lymphoma-associated antigen 2
UniProt Protein Name
cTAGE family member 2
Protein Family
UniProt Gene Name
CTAGE1
UniProt Synonym Gene Names
CTAGE2; Protein cTAGE-2; CT21.2
UniProt Entry Name
CTGE2_HUMAN
Uniprot Description
Subcellular location: Membrane; Single-pass membrane protein
Potential.
Tissue specificity: Testis. Not found in tumor.
Sequence similarities: Belongs to the cTAGE family.
Sequence caution: The sequence AAK63198.1 differs from that shown. Reason: Frameshift at position 741.
Research Articles on CTAGE1
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Product Notes
The CTAGE1 ctage1 (Catalog #AAA8102571) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CTAGE1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CTAGE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:1000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the CTAGE1 ctage1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CTAGE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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