Mouse anti-Human Fetuin A Monoclonal Antibody | anti-AHSG antibody

Anti-Fetuin A Antibody, Mouse Monoclonal

Cat. Num. AAA8102246

• Gene Names: AHSG; AHS; A2HS; HSGA; FETUA
• Reactivity: Human
• Applications: ELISA, ELISA
• Purity: Protein A
• Synonyms: Fetuin A; Monoclonal Antibody; Anti-Fetuin A Antibody; Mouse Monoclonal; Fetuin-A/AHSG Antibody; Mouse MAb; alpha-2-HS-glycoprotein; Anti-A2HS Antibody; Anti-AHS Antibody; Anti-FETUA Antibody; Anti-HSGA Antibody; anti-AHSG antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 6C8E9A10
• Specificity: Human Fetuin-A/AHSG
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-AHSG antibody: ELISA (EIA), ELISA (EIA) (Cap)
• Application Notes: ELISA: 1:1000-1:2000; ELISA (Cap): 1:250-1:2000; ELISA (Cap): This antibody will detect Human Fetuin-A in ELISA pair set.; In a sandwich ELISA, it can be used as capture antibody when paired with.
• Immunogen: Human cell-derived Fetuin-A protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Fetuin-A/AHSG (rhFetuin-A). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-AHSG antibody

Fetuin-A, also known as Alpha-2-HS-Glycoprotein (AHSG), belongs to the Fetuin family, is a plasma binding protein, and is more abundant in fetal than adult blood. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuins are carrier proteins like albumin. Fetuin-A forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate. Thus Fetuin-A is a potent inhibitor of pathological calcification. The circulating levels of fetuin-A, a well-described inhibitor of calcification, regulate the cell-dependent process of osteogenesis. The low circulating fetuin-A levels are associated with a greater prevalence and/or severity of Vascular calcification (VC) and increased risk for all-cause and cardiovascular mortality. However, high circulating fetuin-A levels appear to induce insulin resistance and, in non-dialyzed subjects with diabetic nephropathy, are directly related to VC burden. The emerging role of fetuin-A deficiency as a risk factor in dialysis patients was documented in cross-sectional studies demonstrating a significant correlation with all-cause and cardiovascular mortality. Additionally, Human fetuin-A is a negative acute phase protein involved in inflammatory diseases, thus being a potential physiological regulator of meprin activity. Fetuin-A is a broad-range protease inhibitor. Fetuin-A and cystatin C as endogenous proteolytic regulators of meprin activity broadens our understanding of the proteolytic network in plasma.

References

Mehrotra R. (2007) Emerging role for fetuin-A as contributor to morbidity and mortality in chronic kidney disease. Kidney Int. 72(2): 137-40.Westenfeld R, et al. (2007) Vascular calcification and fetuin-A deficiency in chronic kidney disease. Trends Cardiovasc Med. 17(4): 124-8.Heiss A, et al. (2008). Hierarchical role of fetuin-A and acidic serum proteins in the formation and stabilization of calcium phosphate particles. J Biol Chem. 283 (21): 14815-25.Jahnen-Dechent W, et al. (2008). Mineral chaperones: a role for fetuin-A and osteopontin in the inhibition and regression of pathologic calcification. J Mol Med. 86 (4): 379-89.Hedrich J, et al. (2010) Fetuin-A and cystatin C are endogenous inhibitors of human meprin metalloproteases. Biochemistry. 49(39): 8599-607.

NCBI and Uniprot Product Information

• NCBI GI #: 156523970
• NCBI GeneID: 197
• NCBI Accession #: NP_001613.2
• NCBI GenBank Nucleotide #: NM_001622.2
• UniProt Accession #: P02765; O14961; O14962; Q9P152; A8K9N6; B2R7G1; AAB29984
• Molecular Weight: 49,000
• NCBI Official Full Name: alpha-2-HS-glycoprotein preproprotein
• NCBI Official Synonym Full Names: alpha-2-HS-glycoprotein
• NCBI Official Symbol: AHSG
• NCBI Official Synonym Symbols: AHS; A2HS; HSGA; FETUA
• NCBI Protein Information: alpha-2-HS-glycoprotein; fetuin-A; alpha-2-Z-globulin; ba-alpha-2-glycoprotein
• UniProt Protein Name: Alpha-2-HS-glycoprotein
• Protein Family: Alpha-2-HS-glycoprotein
• UniProt Gene Name: AHSG
• UniProt Synonym Gene Names: FETUA
• UniProt Entry Name: FETUA_HUMAN

NCBI Description

Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure. [provided by RefSeq, Jul 2008]

Uniprot Description

FETUA: Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. Alpha-2-HS glycoprotein derives from this precursor, when the connecting peptide is cleaved off. The two chains A and B are held together by a single disulfide bond. Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. Belongs to the fetuin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space; extracellular region

Molecular Function: kinase inhibitor activity; cysteine protease inhibitor activity

Biological Process: negative regulation of bone mineralization; pinocytosis; ossification; positive regulation of phagocytosis; regulation of inflammatory response; acute-phase response; negative regulation of insulin receptor signaling pathway; negative regulation of phosphorylation; regulation of bone mineralization; skeletal development