Rabbit anti-Human APOBEC3B Polyclonal Antibody | anti-APOBEC3B antibody

Anti-APOBEC3B Antibody, Rabbit Polyclonal

Cat. Num. AAA8102098

• Gene Names: APOBEC3B; A3B; ARP4; ARCD3; PHRBNL; APOBEC1L; bK150C2.2; DJ742C19.2
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: APOBEC3B; Polyclonal Antibody; Anti-APOBEC3B Antibody; Rabbit Polyclonal; APOBEC3B Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-A3B Antibody; Anti-APOBEC1L Antibody; Anti-ARCD3 Antibody; Anti-ARP4 Antibody; Anti-bK150C2.2 Antibody; Anti-CTA-150C2.15-003 Antibody; Anti-DJ742C19.2 Antibody; Anti-PHRBNL Antibody; anti-APOBEC3B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human APOBEC3B
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-APOBEC3B antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 1-4uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human APOBEC3B
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human APOBEC3B, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-APOBEC3B rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:46 kDaObserved band size:50 kDa(We are unsure as to the identity of these extra bands.))

Immunoprecipitation (IP)

(APOBEC3B was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate4 uL anti-APOBEC3B rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-APOBEC3B rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 46 kDaObserved band size: 46 kDa)

Related Product Information for anti-APOBEC3B antibody

The APOBEC3 DNA cytosine deaminase family comprises a fundamental arm of the innate immune response and is best known for retrovirus restriction. Several APOBEC3 enzymes restrict HIV-1 and related retroviruses by deaminating viral cDNA cytosines to uracils compromising viral genomes. Human APOBEC3B (A3B) shows strong virus restriction activities in a variety of experimental systems, and is subjected to tight post-translational regulation evidenced by cell-specific HIV-1 restriction activity and active nuclear import. The APOBEC3B (A3B) single-stranded DNA (ssDNA) cytosine deaminase has important roles in innate immunity but is also a major endogenous source of mutations in cancer. APOBEC3 (A3B) protein families, a DNA cytidine deaminase, were up-regulated in multiple tumors. A3B is essential for oncogenesis of HCC, and is a potential target for preventive intervention. A common large deletion in the APOBEC3 cluster (the APOBEC3B deletion) may increase the risk of breast cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 393715117
• NCBI GeneID: 9582
• NCBI Accession #: NP_004891
• NCBI GenBank Nucleotide #: NM_004900.4
• UniProt Accession #: Q9UH17; O95618; Q20WL1; Q5IFJ4; Q7Z2N3; Q7Z6D6; Q9UE74; B0QYD2
• Molecular Weight: Predicted: 42 kDa; ; Observed: 48 kDa
• NCBI Official Full Name: DNA dC->dU-editing enzyme APOBEC-3B isoform a
• NCBI Official Synonym Full Names: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B
• NCBI Official Symbol: APOBEC3B
• NCBI Official Synonym Symbols: A3B; ARP4; ARCD3; PHRBNL; APOBEC1L; bK150C2.2; DJ742C19.2
• NCBI Protein Information: DNA dC->dU-editing enzyme APOBEC-3B; phorbolin 2; phorbolin 3; phorbolin-2/3; cytidine deaminase; phorbolin-1-related protein; probable DNA dC->dU-editing enzyme APOBEC-3B; apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3B
• UniProt Protein Name: DNA dC->dU-editing enzyme APOBEC-3B
• Protein Family: DNA dC->dU-editing enzyme
• UniProt Gene Name: APOBEC3B
• UniProt Synonym Gene Names: A3B
• UniProt Entry Name: ABC3B_HUMAN

NCBI Description

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. A hybrid gene results from the deletion of approximately 29.5 kb of sequence between this gene, APOBEC3B, and the adjacent gene APOBEC3A. The breakpoints of the deletion are within the two genes, so the deletion allele is predicted to have the promoter and coding region of APOBEC3A, but the 3' UTR of APOBEC3B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

APOBEC3B: a DNA cytidine deaminase involved in foreign DNA clearance. Acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single-or double-stranded RNA. Exhibits antiviral activity against simian immunodeficiency virus (SIV), hepatitis B virus (HBV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Homodimer. Interacts with APOBEC3G. Does not interact with APOBEC1. Expressed at high and moderate levels in peripheral blood leukocytes, spleen, testes, heart, thymus, prostate and ovary. Also expressed at low levels in several other tissues. Phorbol 12-myristate 13-acetate (PMA) induces overexpression in keratinocytes. Up-regulated by IFN-alpha. Belongs to the cytidine and deoxycytidylate deaminase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; RNA-binding; EC 3.5.4.-

Chromosomal Location of Human Ortholog: 22q13.1-q13.2

Cellular Component: nucleus

Molecular Function: zinc ion binding; deoxycytidine deaminase activity

Biological Process: metabolic process; innate immune response; defense response to virus