Rabbit acyl-CoA Thioesterase 2 Polyclonal Antibody | anti-ACOT2 antibody

Anti-acyl-CoA Thioesterase 2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102067

• Gene Names: ACOT2; MTE1; PTE2; CTE1A; PTE2A; CTE-IA; ZAP128
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: acyl-CoA Thioesterase 2; Polyclonal Antibody; Anti-acyl-CoA Thioesterase 2 Antibody; Rabbit Polyclonal; ACOT2 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-CTE-IA Antibody; Anti-CTE1A Antibody; Anti-MTE1 Antibody; Anti-PTE2 Antibody; Anti-PTE2A Antibody; Anti-ZAP128 Antibody; anti-ACOT2 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human acyl-CoA Thioesterase 2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ACOT2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human ACOT2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human ACOT2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ACOT2 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDaObserved band size:49 kDa)

Related Product Information for anti-ACOT2 antibody

Acyl-CoA thioesterases (ACOTs) catalyze the hydrolysis of CoA esters to free CoA and carboxylic acids and have important functions in lipid metabolism and other cellular processes. It's reported that the crystal structure at 2.1A resolution of human mitochondrial ACOT2, a type I enzyme. The structure contains two domains, N and C domains. The C domain has the alpha/beta hydrolase fold, with the catalytic triad Ser294-His422-Asp388. The N domain contains a seven-stranded beta-sandwich, which has some distant structural homologs in other proteins. The active site is located in a large pocket at the interface between the two domains. The structural information has significant relevance for other type I ACOTs and related enzymes.

NCBI and Uniprot Product Information

• NCBI GI #: 148727286
• NCBI GeneID: 10965
• NCBI Accession #: NP_006812.3
• NCBI GenBank Nucleotide #: NM_006821.5
• UniProt Accession #: P49753; Q3I5F8; Q53EK4; Q9NUX4
• Molecular Weight: 53,218 Da
• NCBI Official Full Name: acyl-coenzyme A thioesterase 2, mitochondrial
• NCBI Official Synonym Full Names: acyl-CoA thioesterase 2
• NCBI Official Symbol: ACOT2
• NCBI Official Synonym Symbols: MTE1; PTE2; CTE1A; PTE2A; CTE-IA; ZAP128
• NCBI Protein Information: acyl-coenzyme A thioesterase 2, mitochondrial; long-chain acyl-CoA thioesterase 2; mitochondrial acyl-CoA thioesterase 1; acyl-coenzyme A thioester hydrolase 2a; peroxisomal long-chain acyl-coA thioesterase 2
• UniProt Protein Name: Acyl-coenzyme A thioesterase 2, mitochondrial
• Protein Family: Acyl-CoA thioesterase
• UniProt Gene Name: ACOT2
• UniProt Synonym Gene Names: PTE2; PTE2A; Acyl-CoA thioesterase 2
• UniProt Entry Name: ACOT2_HUMAN

NCBI Description

This gene encodes a member of the acyl-CoA thioesterase protein family, and is one of four acyl-CoA hydrolase genes located in a cluster on chromosome 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

ACOT2: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Displays high levels of activity on medium- and long chain acyl CoAs. Belongs to the C/M/P thioester hydrolase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.2.2; Hydrolase; Mitochondrial; Lipid Metabolism - unsaturated fatty acid biosynthesis

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: mitochondrion

Molecular Function: acyl-CoA hydrolase activity; palmitoyl-CoA hydrolase activity; receptor binding

Biological Process: acyl-CoA metabolic process; very-long-chain fatty acid metabolic process; long-chain fatty acid metabolic process