Rabbit Alpha-Tubulin Polyclonal Antibody | anti-TUBA1A antibody

Alpha-Tubulin Loading Control Antibody, Rabbit PAb, Antigen Affinity Purified

Cat. Num. AAA8101772

• Gene Names: TUBA1A; LIS3; TUBA3; B-ALPHA-1
• Reactivity: Human; Predicted to React with: Mouse, Rat, Rabbit, Rhesus, Pig, Chinese hamster, Bovine, Dog (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Alpha-Tubulin; Polyclonal Antibody; Alpha-Tubulin Loading Control Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-B-ALPHA-1 Antibody; Anti-LIS3 Antibody; Anti-TUBA3 Antibody; anti-TUBA1A antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat, Rabbit, Rhesus, Pig, Chinese hamster, Bovine, Dog (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human TBA1A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-TUBA1A antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:500-1:2000; ICC/IF: 1:100-1:500; IP: 1-5uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human Alpha-Tubulin
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Alpha-Tubulin, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-Alpha-Tubulin rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:50 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human Alpha-Tubulin in human prostate with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human Alpha-Tubulin in human testis with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of Alpha-Tubulin in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human Alpha-Tubulin polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Immunoprecipitation (IP)

(Alpha-Tubulin was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate 4 uL anti-Alpha-Tubulin rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Alpha-Tubulin rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 55 kDa Observed band size :55 kDa)

Related Product Information for anti-TUBA1A antibody

TUBA1A is a highly-conserved gene with few changes among eukaryotes and few polymorphic variants in human populations. TUBA1A encodes the tubulin alpha-1A chain, a protein that is highly expressed in the cerebral cortex, hippocampus, cerebellum, and brainstem of the developing fetal brain, with a decrease in postnatal and adult stages. Alpha-and beta-tubulin subunits form dimers, which then co-assemble into microtubule polymers: dynamic, scaffold-like structures that perform key functions during neurogenesis, neuronal migration, and cortical organization. Microtubules are dynamic polymers that perform a range of mechanical tasks within the cell. Mutations in TUBA1A have been reported to cause a range of brain malformations.

References

Gardner JF, et al. (2018) Clinical and functional characterization of the recurrent tuba1a p.(arg2his) mutation. Brain Sci 8 (8): 145.

NCBI and Uniprot Product Information

• NCBI GI #: 55977864
• NCBI GeneID: 7846
• UniProt Accession #: Q71U36; P04687; P05209; A8K0B8; G3V1U9
• Molecular Weight: 46,297 Da
• NCBI Official Full Name: Tubulin alpha-1A chain
• NCBI Official Synonym Full Names: tubulin, alpha 1a
• NCBI Official Symbol: TUBA1A
• NCBI Official Synonym Symbols: LIS3; TUBA3; B-ALPHA-1
• NCBI Protein Information: tubulin alpha-1A chain; hum-a-tub1; hum-a-tub2; tubulin B-alpha-1; tubulin alpha-3 chain; tubulin, alpha, brain-specific
• UniProt Protein Name: Tubulin alpha-1A chain
• Protein Family: Alpha-tubulin N-acetyltransferase
• UniProt Gene Name: TUBA1A
• UniProt Synonym Gene Names: TUBA3
• UniProt Entry Name: TBA1A_HUMAN

NCBI Description

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

TUBA1A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Expressed at a high level in fetal brain. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: microtubule; recycling endosome; cytoplasmic microtubule; cytosol; nucleus

Molecular Function: GTPase activity; protein domain specific binding; protein binding; GTP binding; structural constituent of cytoskeleton; structural molecule activity

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; cell division; organelle organization and biogenesis; cytoskeleton-dependent intracellular transport; mitotic cell cycle; G2/M transition of mitotic cell cycle; microtubule-based process

Disease: Lissencephaly 3