Rabbit anti-Human ZAP70 Polyclonal Antibody | anti-ZAP70 antibody

Anti-ZAP70 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101765

• Gene Names: ZAP70; SRK; STD; TZK; STCD; ZAP-70
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: ZAP70; Polyclonal Antibody; Anti-ZAP70 Antibody; Rabbit Polyclonal; ZAP70 Antibody; Rabbit PAb; Antigen Affinity Purified; zeta-chain (TCR) associated protein kinase 70kDa; Anti-SRK Antibody; Anti-STCD Antibody; Anti-STD Antibody; Anti-TZK Antibody; Anti-ZAP-70 Antibody; anti-ZAP70 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ZAP70
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ZAP70 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 1-2uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the center region of the Human ZAP70
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ZAP70, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(ZAP70 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate1 uL anti-ZAP70 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ZAP70 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 70 kDaObserved band size: 70 kDa)

Western Blot (WB)

(Anti-ZAP70 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:70 kDaObserved band size:70 kDa)

Related Product Information for anti-ZAP70 antibody

The ZAP70 (zeta-associated protein of 70 kDa) is expressed in the aggressive form of B-cell chronic lymphocytic leukemia (CLL). ZAP70 plays a critical role for ZAP70 in chemokine-mediated, inside-out signaling to integrins in trisomy. The phosphorylation of ZAP70 activated the downstream NF-kappaB signaling pathway, which is involved in both T cell growth and function. On the other hand, the expression of Zap70 in B-cell malignancies correlates with the severity of the disease. Because of its role in immune-related disorders, Zap70 has become a therapeutic target for the treatment of human diseases.

Product Categories/Family for anti-ZAP70 antibody

NF-kB (NFkB) Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 31455611
• NCBI GeneID: 7535
• NCBI Accession #: NP_001070.2
• NCBI GenBank Nucleotide #: NM_001079.3
• UniProt Accession #: P43403; Q6PIA4; Q8IXD6; Q9UBS6; A6NFP4
• Molecular Weight: 69,872 Da
• NCBI Official Full Name: tyrosine-protein kinase ZAP-70 isoform 1
• NCBI Official Synonym Full Names: zeta-chain (TCR) associated protein kinase 70kDa
• NCBI Official Symbol: ZAP70
• NCBI Official Synonym Symbols: SRK; STD; TZK; STCD; ZAP-70
• NCBI Protein Information: tyrosine-protein kinase ZAP-70; syk-related tyrosine kinase; 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; zeta-chain associated protein kinase, 70kD
• UniProt Protein Name: Tyrosine-protein kinase ZAP-70
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ZAP70
• UniProt Synonym Gene Names: SRK
• UniProt Entry Name: ZAP70_HUMAN

NCBI Description

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ZAP70: a tyrosine kinase of the Syk family. Associates with the T-cell antigen receptor zeta-chain after TCR stimulation. Phosphorylated by Src-family kinases following antigen receptor activation. Plays a role in lymphocyte activation.

Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; EC 2.7.10.2; TK group; Syk family

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; immunological synapse; intercellular junction; cytosol; lipid raft

Molecular Function: protein binding; phosphotyrosine binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: cell migration; adaptive immune response; peptidyl-tyrosine phosphorylation; B cell activation; T cell activation; positive regulation of calcium-mediated signaling; negative thymic T cell selection; protein amino acid phosphorylation; T cell receptor signaling pathway; positive regulation of T cell differentiation; innate immune response; immune response; positive regulation of alpha-beta T cell proliferation; beta selection; positive regulation of alpha-beta T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; positive thymic T cell selection

Disease: Selective T-cell Defect