Rabbit FOXP2 Polyclonal Antibody | anti-FOXP2 antibody

Anti-FOXP2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101744

• Gene Names: FOXP2; SPCH1; CAGH44; TNRC10
• Reactivity: Human; Predicted to React with: Mouse, Rat, Rhesus (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: FOXP2; Polyclonal Antibody; Anti-FOXP2 Antibody; Rabbit Polyclonal; FOXP2 Antibody; Rabbit PAb; Antigen Affinity Purified; forkhead box P2; Anti-CAGH44 Antibody; Anti-SPCH1 Antibody; Anti-TNRC10 Antibody; anti-FOXP2 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat, Rhesus (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human FOXP2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-FOXP2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; ICC/IF: 1:1000-1:5000
• Immunogen: A synthetic peptide corresponding to the center region of the Human FOXP2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human FOXP2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-FOXP2 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: HEK293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:80 kDaObserved band size:70 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of FOXP2 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human FOXP2 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red).Positive staining was localized to nucleus.)

Related Product Information for anti-FOXP2 antibody

Forkhead box (FOX) proteins are a ubiquitously expressed family of transcription factors that regulate the development and differentiation of a wide range of tissues in animals. The FOXP subfamily members are the only known FOX proteins capable of forming domain-swapped forkhead domain (FHD) dimers. The change in dynamics of the hinge-loop region of FOXP2 alters the energetics and mechanism of DNA binding highlighting the critical role of hinge loop mutations in regulating DNA binding characteristics of the FOX proteins. FOXP2 is a transcription factor expressed in multiple tissues during embryonic development. FOXP2 regulates transcription by binding to DNA at its DNA binding domain, the forkhead domain (FHD) through the recognition helix. FOXP2 as a novel suppressor and prognostic marker of breast cancer metastasis. FOXP2 encodes a transcription factor involved in speech and language acquisition. Mutations of the FOXP2 gene cause a severe speech and language disorder, providing a molecular window into the neurobiology of language.

NCBI and Uniprot Product Information

• NCBI GI #: 289666732
• NCBI GeneID: 93986
• NCBI Accession #: NP_001166237.1
• NCBI GenBank Nucleotide #: NM_001172766.2
• UniProt Accession #: O15409; Q6ZND1; Q75MJ3; Q8IZE0; Q8N0W2; Q8N6B7; Q8N6B8; Q8NFQ1; A0AUV6; A4D0U8; A6NNW4; B4DLD9
• Molecular Weight: 81,840 Da
• NCBI Official Full Name: forkhead box protein P2 isoform V
• NCBI Official Synonym Full Names: forkhead box P2
• NCBI Official Symbol: FOXP2
• NCBI Official Synonym Symbols: SPCH1; CAGH44; TNRC10
• NCBI Protein Information: forkhead box protein P2; CAG repeat protein 44; forkhead/winged-helix transcription factor; trinucleotide repeat containing 10; trinucleotide repeat-containing gene 10 protein
• UniProt Protein Name: Forkhead box protein P2
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXP2
• UniProt Synonym Gene Names: CAGH44; TNRC10
• UniProt Entry Name: FOXP2_HUMAN

NCBI Description

This gene encodes a member of the forkhead/winged-helix (FOX) family of transcription factors. It is expressed in fetal and adult brain as well as in several other organs such as the lung and gut. The protein product contains a FOX DNA-binding domain and a large polyglutamine tract and is an evolutionarily conserved transcription factor, which may bind directly to approximately 300 to 400 gene promoters in the human genome to regulate the expression of a variety of genes. This gene is required for proper development of speech and language regions of the brain during embryogenesis, and may be involved in a variety of biological pathways and cascades that may ultimately influence language development. Mutations in this gene cause speech-language disorder 1 (SPCH1), also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Multiple alternative transcripts encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]

Uniprot Description

FOXP2: Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Involved in neural mechanisms mediating the development of speech and language. Forms homodimers and heterodimers with FOXP1 and FOXP4. Dimerization is required for DNA-binding. Interacts with CTBP1. Isoform 1 and isoform 6 are expressed in adult and fetal brain, caudate nucleus and lung. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; metal ion binding; transcription factor activity

Biological Process: skeletal muscle development; caudate nucleus development; camera-type eye development; transcription, DNA-dependent; righting reflex; putamen development; negative regulation of transcription from RNA polymerase II promoter; vocal learning; post-embryonic development; smooth muscle development; positive regulation of mesenchymal cell proliferation; cerebellum development; cerebral cortex development; negative regulation of transcription, DNA-dependent; alveolus development; growth

Disease: Speech-language Disorder 1