Rabbit MEK5/MAP2K5 Polyclonal Antibody | anti-MAP2K5 antibody

Anti-MEK5/MAP2K5 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100825

• Gene Names: MAP2K5; MEK5; MAPKK5; PRKMK5; HsT17454
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: MEK5/MAP2K5; Polyclonal Antibody; Anti-MEK5/MAP2K5 Antibody; Rabbit Polyclonal; MAP2K5 Antibody; Rabbit PAb; Antigen Affinity Purified; mitogen-activated protein kinase kinase 5; Anti-HsT17454 Antibody; Anti-MAPKK5 Antibody; Anti-MEK5 Antibody; Anti-PRKMK5 Antibody; anti-MAP2K5 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MEK5/MAP2K5
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MAP2K5 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:100-1:500; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human MAP2K5
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human MAP2K5, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of MAP2K5 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human MAP2K5 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.)

Immunohistochemistry (IHC)

(Immunochemical staining of human MAP2K5 in human skeletal muscle with rabbit polyclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human MAP2K5 in human heart with rabbit polyclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-MAP2K5 antibody

MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5, also known as MEK5) is a Protein Coding gene. The protein encoded by this gene is a dual-specificity protein kinase that belongs to the MAP kinase kinase family. The signal cascade mediated by this kinase is involved in growth factor-stimulated cell proliferation and muscle cell differentiation. 4 alternatively spliced human isoforms have been reported. Diseases associated with MAP2K5 include Caroli Disease and Restless Legs Syndrome. SNP rs1026732 in MAP2K5/SKOR1 was significantly associated with restless legs syndrome (RLS). RLS is considered a genetic disease and, following a genome-wide association study conducted in 2007, the MAP2K5 gene has been regarded as the promising candidate gene for RLS.

Product Categories/Family for anti-MAP2K5 antibody

Death Receptor Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 332164798
• NCBI GeneID: 5607
• NCBI Accession #: NP_001193733.1
• NCBI GenBank Nucleotide #: NM_001206804.1
• UniProt Accession #: Q13163; Q92961; Q92962; B4DE43
• Molecular Weight: 50112 MW
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 5 isoform C
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 5
• NCBI Official Symbol: MAP2K5
• NCBI Official Synonym Symbols: MEK5; MAPKK5; PRKMK5; HsT17454
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 5
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 5
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K5
• UniProt Synonym Gene Names: MEK5; MKK5; PRKMK5; MAP kinase kinase 5; MAPKK 5; MEK 5

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq, May 2011]

Uniprot Description

Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.