Rabbit EAAT1/SLC1A3 Polyclonal Antibody | anti-SLC1A3 antibody

Anti-EAAT1/SLC1A3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100616

• Gene Names: Slc1a3; Gmt1; Eaat1; GLAST; GLU-T; GluT-1; MGluT1; GLAST-1; AI504299; B430115D02Rik
• Reactivity: Mouse; Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: EAAT1/SLC1A3; Polyclonal Antibody; Anti-EAAT1/SLC1A3 Antibody; Rabbit Polyclonal; SLC1A3 Antibody; Rabbit PAb; Antigen Affinity Purified; solute carrier family 1 (glial high affinity glutamate transporter); member 3; Anti-AI504299 Antibody; Anti-B430115D02Rik Antibody; Anti-Eaat1 Antibody; Anti-GLAST Antibody; Anti-GLAST-1 Antibody; Anti-GLU-T Antibody; Anti-GluT-1 Antibody; Anti-Gmt1 Antibody; Anti-MGluT1 Antibody; anti-SLC1A3 antibody

• Host: Rabbit
• Reactivity: Mouse; Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse EAAT1/SLC1A3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SLC1A3 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:1000-1:4000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the MOUSE SLC1A3
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the MOUSE SLC1A3, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human Slc1a3 in human brain with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-SLC1A3 antibody

The SLC1A3 (Solute Carrier Family 1, member 3) gene encodes a glial glutamate transporter-excitatory amino-acid transporter 1, which is a trimeric transmembrane protein responsible for glutamate transport in the synaptic cleft and maps to chromosome 5p12, a region of linkage that coincides in two published ADHD genome scans so far. SLC1A3 is thus both a functional and positional candidate gene for ADHD. Episodic ataxia type 6 (EA6) is caused by mutations in SLC1A3 that encodes excitatory amino acid transporter 1 (EAAT1), a glial glutamate transporter. EAAT1 regulates the extent and duration of the glutamate-mediated signal by the clearance of glutamate after synaptic release. Besides, EAAT1 also has an anion channel activity that prevents additional glutamate release. p53 promotes the expression of SLC1A3, which allows the utilization of aspartate to support cells in the absence of extracellular glutamine.

References

Turic D, et al. (2005) A family based study implicates solute carrier family 1-member 3 (slc1a3) gene in attention-deficit/hyperactivity disorder. Biol Psychiatry 57 (11): 1461-1466.Iwama K, et al. (2018) A novel mutation in slc1a3 causes episodic ataxia. J Hum Genet 63 (2): 207-211.Choi KD, et al. (2017) Late-onset episodic ataxia associated with slc1a3 mutation. J Hum Genet 62 (3): 443-446.

NCBI and Uniprot Product Information

• NCBI GI #: 24233554
• NCBI GeneID: 20512
• NCBI Accession #: NP_683740.1
• NCBI GenBank Nucleotide #: NM_148938.3
• UniProt Accession #: P56564; Q99P53
• Molecular Weight: 59,622 Da
• NCBI Official Full Name: excitatory amino acid transporter 1
• NCBI Official Synonym Full Names: solute carrier family 1 (glial high affinity glutamate transporter), member 3
• NCBI Official Symbol: Slc1a3
• NCBI Official Synonym Symbols: Gmt1; Eaat1; GLAST; GLU-T; GluT-1; MGluT1; GLAST-1; AI504299; B430115D02Rik
• NCBI Protein Information: excitatory amino acid transporter 1
• UniProt Protein Name: Excitatory amino acid transporter 1
• Protein Family: Excitatory amino acid transporter
• UniProt Gene Name: Slc1a3
• UniProt Synonym Gene Names: Eaat1; Gmt1; GLAST-1
• UniProt Entry Name: EAA1_MOUSE

Uniprot Description

SLC1A3: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial; Transporter; Transporter, SLC family

Cellular Component: basolateral plasma membrane; cell projection; cell soma; cell surface; dendritic spine; fibril; integral to membrane; membrane; mitochondrial inner membrane; neuron projection; plasma membrane; synapse

Molecular Function: amino acid binding; glutamate binding; high-affinity glutamate transmembrane transporter activity; L-glutamate transmembrane transporter activity; protein binding; sodium:dicarboxylate symporter activity; symporter activity

Biological Process: auditory behavior; cranial nerve development; dicarboxylic acid transport; gamma-aminobutyric acid biosynthetic process; glutamate biosynthetic process; glutamate metabolic process; L-glutamate import; L-glutamate transport; malate-aspartate shuttle; neuromuscular process controlling balance; neuron morphogenesis during differentiation; positive regulation of defense response to virus by host; positive regulation of synaptic transmission; response to amino acid stimulus; response to antibiotic; response to drug; response to inorganic substance; response to light stimulus; response to wounding; sensory perception of sound; transport