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Western Blot (WB) (Anti-CXCR5 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MOLT-4 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:42 kDaObserved band size:42 kDa)

Rabbit anti-Human CXCR5 Polyclonal Antibody | anti-CXCR5 antibody

Anti-CXCR5 Antibody, Rabbit Polyclonal

Gene Names
CXCR5; BLR1; CD185; MDR15
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
CXCR5; Polyclonal Antibody; Anti-CXCR5 Antibody; Rabbit Polyclonal; CXCR5 (CD185) Antibody; Rabbit PAb; Antigen Affinity Purified; chemokine (C-X-C motif) receptor 5; Anti-BLR1 Antibody; Anti-CD185 Antibody; Anti-MDR15 Antibody; anti-CXCR5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CXCR5 (CD185)
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CXCR5 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to the N-terminus of the human CXCR5 (CD185)
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, a synthetic peptide corresponding to the N-terminus of the human CXCR5 (CD185). Human CXCR5 (CD185) specific IgG was purified by Human CXCR5 (CD185) affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CXCR5 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MOLT-4 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:42 kDaObserved band size:42 kDa)

Western Blot (WB) (Anti-CXCR5 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MOLT-4 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:42 kDaObserved band size:42 kDa)
Related Product Information for anti-CXCR5 antibody
Because of the level of attention it received due to its role as the principal HIV coreceptor, CCR5 has been described as a 'celebrity' chemokine receptor. The wealth of CCR5-related tools that have been developed during the intensive investigation of CCR5 as an HIV drug target can now be turned towards the study of CCR5 as a model chemokine receptor. The enforced expression of CXCR5 onto Treg cells efficiently induces Tfr cell-like properties, which might be a promising cellular therapeutic approach for the treatment of antibody-mediated autoimmune diseases. The Chemokine receptor 5 (CCR5) is implicated in immune cell migration and cytokine release in the CNS, and it was demonstrated to strongly contribute to CNS inflammation and damage in several models of sterile and pathogen-mediated CNS diseases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
643
UniProt Accession #
Molecular Weight
36,676 Da
NCBI Official Full Name
C-X-C chemokine receptor type 5
NCBI Official Synonym Full Names
chemokine (C-X-C motif) receptor 5
NCBI Official Symbol
CXCR5
NCBI Official Synonym Symbols
BLR1; CD185; MDR15
NCBI Protein Information
C-X-C chemokine receptor type 5; CXC-R5; CXCR-5; MDR-15; monocyte-derived receptor 15; Burkitt lymphoma receptor 1, GTP-binding protein; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5)
UniProt Protein Name
C-X-C chemokine receptor type 5
Protein Family
UniProt Gene Name
CXCR5
UniProt Synonym Gene Names
BLR1; MDR15; CXC-R5; CXCR-5; MDR-15
UniProt Entry Name
CXCR5_HUMAN

NCBI Description

This gene encodes a multi-pass membrane protein that belongs to the CXC chemokine receptor family. It is expressed in mature B-cells and Burkitt's lymphoma. This cytokine receptor binds to B-lymphocyte chemoattractant (BLC), and is involved in B-cell migration into B-cell follicles of spleen and Peyer patches. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

CXCR5: Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; C-X-C chemokine receptor activity

Biological Process: positive regulation of cytokinesis; G-protein coupled receptor protein signaling pathway; B cell activation; immune response; cell motility; chemotaxis; lymph node development

Research Articles on CXCR5

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Product Notes

The CXCR5 cxcr5 (Catalog #AAA8100326) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CXCR5 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CXCR5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the CXCR5 cxcr5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CXCR5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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