Mouse anti-Human Beta-Tubulin Monoclonal Antibody | anti-Beta-Tubulin antibody

Beta-Tubulin Loading Control Antibody, Mouse MAb

Cat. Num. AAA8100087

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A
• Synonyms: Beta-Tubulin; Monoclonal Antibody; Beta-Tubulin Loading Control Antibody; Mouse MAb; anti-Beta-Tubulin antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 05
• Specificity: Human Beta-Tubulin
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-Beta-Tubulin antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000-1:10,0000; IHC-P: 1:2000-1:20,000; ICC/IF: 1:50-1:200; IP: 5-10uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the N-terminus of the human beta-tubulin
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the human beta-tubulin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human Tubulin in human breast carcinoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human Tubulin in human malignant melanoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human Tubulin in human prostate with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of Human Beta-Tubulin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human Beta-Tubulin monoclonal antibody at 4 degree C overnight. Then cells were stained with the Alexa Fluor 594-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor488-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoskeleton.)

Western Blot (WB)

(Anti-Beta-Tubulin mouse monoclonal antibody at 1:10000, 1:20000, 1:50000, 1:100000 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: Raw246.7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)

Western Blot (WB)

(Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution Lane A: Rabbit heart tissue lysate Lane B: Rabbit liver tissue lysate Lane C: Rabbit spleen tissue lysate Lane D: Rabbit lung tissue lysate Lane E: Rabbit kidney tissue lysate Lane F: Rabbit brain tissue lysate Lane G: Rabbit muscle tissue lysate Lane H: Rabbit stomach tissue lysate Lane I: Rabbit small intestine tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)

Western Blot (WB)

(Anti-Beta-Tubulin mouse monoclonal antibody at 1:5000 dilution Lane A: Rat heart tissue lysate Lane B: Rat liver tissue lysate Lane C: Rat spleen tissue lysate Lane D: Rat lung tissue lysate Lane E: Rat kidney tissue lysate Lane F: Rat muscle tissue lysate Lane G: Rat stomach tissue lysate Lane H: Rat small intestine tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)

Western Blot (WB)

(Anti-Beta-Tubulin mouse monoclonal antibody at 1:5000 dilution Lane A: Mouse heart tissue lysate Lane B: Mouse liver tissue lysate Lane C: Mouse spleen tissue lysate Lane D: Mouse lung tissue lysate Lane E: Mouse kidney tissue lysate Lane F: Mouse brain tissue lysate Lane G: Mouse muscle tissue lysate Lane H: Mouse stomach tissue lysate Lane I: Mouse pancreas tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)

Western Blot (WB)

(Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution Lane A: Jurkat Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: Hela Whole Cell Lysate Lane E: RAW264.7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)

Western Blot (WB)

(Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Daudi Whole Cell Lysate Lane C: MOLT-4 Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lane E: 293T Whole Cell Lysate Lane F: HelaS3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)