Hsp70 Recombinant Protein | HSPA1A recombinant protein

Recombinant Human HSP70 (native, endotoxin-free)

Cat. Num. AAA806923

• Gene Names: HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A; HEL-S-103
• Applications: Western Blot, SDS-Page, Functional Assay, ELISA
• Purity: Endotoxin-free, Multi-Step Purified
• Synonyms: Hsp70; Recombinant Human HSP70 (native; endotoxin-free); Hsp70 1; Hsp70 2; Hsp70.1; Hsp72; Hsp73; HSPA1; HSPA1A; HSPA1B; HSPA1A recombinant protein

• Host: Human
• Purity/Purification: Endotoxin-free, Multi-Step Purified
• Form/Format: 50mM Tris/HCI pH7.S, 2.SmM Bme, O.lSM NaCl, 10% glycerol
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 641

• Applicable Applications for HSPA1A recombinant protein: Western Blot (WB), SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA (EIA)
• Certificate of Analysis: This product has been certified >90% pure using SDS-PAGE analysis. The protein tested positive for ATPase activity using a Malachite Green assay.
• Conjugate Tag: No tags
• Expression System: Baculovirus/Hi5 insect cells
• Cellular Localization: Cytoplasm
• Research Area(s): Cancer; Heat Shock
• Preparation and Storage: Storage Conditions: -20°C; 1 year+ Avoid freeze/ thaw cycle.; Shipping Temperature: Shipped on cold packs or ambient

SDS-Page

(SDS-PAGE of endotoxin-free 70kDa native human Hsp70 protein (SPR-117).)

Related Product Information for HSPA1A recombinant protein

Scientific Background: Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Product Categories/Family for HSPA1A recombinant protein

Chaperones; Heat Shock; Trafficking

References

1. Zho, J. (1998). Cell 94: 471-480. 2. Boorstein, W. R., Ziegelhoffer, T. & Craig, E. A. (1993), J. Mol. Evol.38 (1) 1-17. 3. Rothman, J. (1989), Cell 59, 591 -601. 4. DeLuca-Flaherty et al. (1990), Cell 62, 875-887. 5. Bork, P., Sander, C. & Valencia, A. (1992), Proc. Nut1 Acad. Sci. USA 89, 7290-7294. 6. Fink, A.L. (1999) Physiol. Rev. 79: 425-449.

NCBI and Uniprot Product Information

• NCBI GI #: 194248072
• NCBI GeneID: 3303
• NCBI Accession #: NP_005336.3
• NCBI GenBank Nucleotide #: NM_005345.5
• UniProt Accession #: P08107; P19790; Q5JQI4; Q5SP17; Q9UQL9; Q9UQM0; B4E3B6
• Molecular Weight: Approximately 70kD
• NCBI Official Full Name: heat shock 70 kDa protein 1A/1B
• NCBI Official Synonym Full Names: heat shock 70kDa protein 1A
• NCBI Official Symbol: HSPA1A
• NCBI Official Synonym Symbols: HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A; HEL-S-103
• NCBI Protein Information: heat shock 70 kDa protein 1A/1B; HSP70-1/HSP70-2; heat shock 70kD protein 1A; heat shock-induced protein; heat shock 70 kDa protein 1/2; epididymis secretory protein Li 103; dnaK-type molecular chaperone HSP70-1
• UniProt Protein Name: Heat shock 70 kDa protein 1A/1B
• Protein Family: HSP70 co-chaperone
• UniProt Gene Name: HSPA1A
• UniProt Synonym Gene Names: HSPA1; HSX70; HSPA1B; HSP70-1/HSP70-2; HSP70.1/HSP70.2
• UniProt Entry Name: HSP71_HUMAN