Rabbit SOD (Cu/Zn) Polyclonal Antibody | anti-Sod1 antibody

SOD (Cu/Zn) Antibody: ATTO 488

Cat. Num. AAA806770

• Gene Names: Sod1; CuZnSOD
• Reactivity: Human, Rat, Mouse, Bovine
• Applications: Western Blot, Immunoprecipitation, ELISA, Immunohistochemistry
• Synonyms: SOD (Cu/Zn); Polyclonal Antibody; SOD (Cu/Zn) Antibody: ATTO 488; Superoxide dismutase1; ALS1; IPOA; SOD1; SOD2; SODC; SOD Antibody; Cu Zn SOD; anti-Sod1 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Bovine
• Clonality: Polyclonal
• Form/Format: Affinity (antigen) Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 154

• Applicable Applications for anti-Sod1 antibody: Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: 0.5ug/ml was sufficient for detection of Cu/Zn SOD in 20ug of rat brain tissue extract
• Conjugate: ATTO 488
• Storage Buffer: PBS pH7.0, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western blot analysis of Human Cell line lysates showing detection of SOD1 protein using Rabbit Anti-SOD1 Polyclonal Antibody (SPC-115). Load: 15 ugprotein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-SOD1 Polyclonal Antibody (SPC-115) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Related Product Information for anti-Sod1 antibody

Background Info: Detects a ~23kDa (human) and 19kDa (other species) proteins corresponding to the molecular mass of Cu/Zn superoxide dismutase (SOD) on SDS PAGE immunoblots.

Scientific Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2" to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).

Product Categories/Family for anti-Sod1 antibody

Oxidative Stress

References

1.Adachi T., et al. (1992). Clin. Chim. Acta. 212: 89-102. 2.Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111- 180. 3.Furukawa Y., O'Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6. 4.Gao B., et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5.Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385. 6.Kurobe N., et al. (1990) Biomedical Research. 11: 187- 194 7.Wispe J.R., et al. (1989) BBA. 994: 30-36. 8.Xiao-Hong Liu., et al. (1993) Brain Research. 625: 29-37.

NCBI and Uniprot Product Information

• NCBI GI #: 8394328
• NCBI GeneID: 24786
• NCBI Accession #: NP_058746.1
• NCBI GenBank Nucleotide #: NM_017050.1
• UniProt Accession #: P07632
• Molecular Weight: 15,912 Da
• NCBI Official Full Name: superoxide dismutase
• NCBI Official Synonym Full Names: superoxide dismutase 1, soluble
• NCBI Official Symbol: Sod1
• NCBI Official Synonym Symbols: CuZnSOD
• NCBI Protein Information: superoxide dismutase [Cu-Zn]; superoxide dismutase [Cu-Zn]
• UniProt Protein Name: Superoxide dismutase [Cu-Zn]
• Protein Family: Superoxide dismutase
• UniProt Gene Name: Sod1
• UniProt Entry Name: SODC_RAT

NCBI Description

catalyzes the conversion of superoxide to hydrogen peroxide and molecular oxygen; involved in response to oxidative stress [RGD, Feb 2006]

Uniprot Description

SOD1: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Homodimer; non-disulfide linked. Homodimerization may take place via the ditryptophan cross-link at Trp-33. The pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 interact with RNF19A, whereas wild-type protein does not. The pathogenic variants ALS1 Arg-86 and Ala-94 interact with MARCH5, whereas wild-type protein does not. Belongs to the Cu-Zn superoxide dismutase family.

Protein type: Mitochondrial; EC 1.15.1.1; Oxidoreductase; Nuclear receptor co-regulator; Apoptosis

Cellular Component: dendrite cytoplasm; extracellular space; neuron projection; protein complex; mitochondrion; cell; extracellular region; mitochondrial intermembrane space; cytosol; secretory granule; nucleoplasm; extracellular matrix; cell soma; cytoplasm; plasma membrane; peroxisome; intracellular; cytoplasmic vesicle; nucleus; myelin sheath

Molecular Function: identical protein binding; enzyme binding; copper ion binding; zinc ion binding; chaperone binding; superoxide dismutase activity; Rac GTPase binding; protein phosphatase 2B binding

Biological Process: positive regulation of catalytic activity; activation of MAPK activity; cellular iron ion homeostasis; myeloid cell homeostasis; retrograde axon cargo transport; response to antibiotic; muscle maintenance; retinal homeostasis; glutathione metabolic process; neurofilament cytoskeleton organization and biogenesis; regulation of mitochondrial membrane potential; positive regulation of superoxide release; negative regulation of neuron apoptosis; response to drug; positive regulation of cytokine production; cell aging; transmission of nerve impulse; response to reactive oxygen species; response to ethanol; response to heat; heart contraction; response to oxidative stress; superoxide release; negative regulation of apoptosis; relaxation of vascular smooth muscle; removal of superoxide radicals; locomotory behavior; response to organic substance; sensory perception of sound; ovarian follicle development; double-strand break repair; regulation of blood pressure; DNA fragmentation during apoptosis; auditory receptor cell stereocilium organization and biogenesis; response to axon injury; anterograde axon cargo transport; negative regulation of cholesterol biosynthetic process; aging; response to nutrient levels; response to superoxide; response to amphetamine; superoxide metabolic process; myelin maintenance in the peripheral nervous system; regulation of multicellular organism growth; response to copper ion; response to hydrogen peroxide; spermatogenesis; regulation of protein kinase activity; hydrogen peroxide biosynthetic process; embryo implantation