Rabbit Hsp90 Polyclonal Antibody | anti-HSP90AB1 antibody

Hsp90 Antibody: FITC

Cat. Num. AAA806191

• Gene Names: HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
• Synonyms: Hsp90; Polyclonal Antibody; Hsp90 Antibody: FITC; Hsp84; Hsp86; Hsp90A; Hsp90AA1; Hsp90AB1; Hsp90B; HspC1; HspC2; HspCAL1; HspCAL4; Hsp90N; anti-HSP90AB1 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Form/Format: Rabbit Antiserum
• Sequence Length: 724

• Applicable Applications for anti-HSP90AB1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: 1:20,000-40,000 (ECL) (WB)
• Conjugate: FITC
• Storage Buffer: Rabbit antiserum
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western blot analysis of Human Cell line lysates showing detection of HSP90 protein using Rabbit Anti-HSP90 Polyclonal Antibody (SPC-104). Load: 2 ugHsp90beta. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP90 Polyclonal Antibody (SPC-104) at 1:10000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-HSP90 Polyclonal Antibody (SPC-104). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP90 Polyclonal Antibody (SPC-104) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.)

Related Product Information for anti-HSP90AB1 antibody

Background Info: Detects ~90kDa proteins corresponding to the molecular mass of Hsp90alpha/beta.

Scientific Background: Hsp90 is a highly conserved and essential stress protein Hsp90 is a highly conserved and essential stress protein

Product Categories/Family for anti-HSP90AB1 antibody

Chaperones; Heat Shock; Trafficking

References

1. Arlander S.J.H., et al. (2003) J Biol Chem 278: 52572-52577. 2. Pearl H., et al. (2001) Adv Protein Chem 59:157-186. 3. Neckers L., et al. (2002) Trends Mol Med 8:S55-S61. 4. Pratt W., Toft D. (2003) Exp Biol Med 228:111-133. 5. Pratt W., Toft D. (1997) Endocr Rev 18: 306-360. 6. Pratt W.B. (1998) Proc Soc Exptl Biol Med 217: 420-434. 7. Whitesell L., et al. (1994) Proc Natl Acad Sci USA 91: 8324- 8328.
1. Wagatsuma, A. et al. (2011). Pharmacological inhibition of HSP90 activity negatively modulates myogenic differentiation and cell survival in C2C12 cells. Mol and Cel Biochem. 358 (1-2), 265-280. doi: 10.1007/s11010-011-0977-0

2. Verheyen, A. et al. (2012). Systemic anti-vascular endothelial growth factor therapies induce a painful sensory neuropathy. Brain. 135 (9), 2629-2641. doi: 10.1093/brain/aws145
3. Zhang, S. et al. (2013). B Cell–Specific Deficiencies in mTOR Limit Humoral Immune Responses. Journal of Immunology. Published online. doi: 10.4049/jimmunol.1201767

NCBI and Uniprot Product Information

• NCBI GI #: 20149594
• NCBI GeneID: 3326
• NCBI Accession #: NP_031381.2
• NCBI GenBank Nucleotide #: NM_007355.3
• UniProt Accession #: P08238; Q5T9W7; Q9NQW0; Q9NTK6; B2R5P0
• Molecular Weight: 83,264 Da
• NCBI Official Full Name: heat shock protein HSP 90-beta isoform a
• NCBI Official Synonym Full Names: heat shock protein 90kDa alpha (cytosolic), class B member 1
• NCBI Official Symbol: HSP90AB1
• NCBI Official Synonym Symbols: HSP84; HSPC2; HSPCB; D6S182; HSP90B
• NCBI Protein Information: heat shock protein HSP 90-beta; HSP90-beta; heat shock 84 kDa; heat shock 90kD protein 1, beta
• UniProt Protein Name: Heat shock protein HSP 90-beta
• Protein Family: Heat shock protein
• UniProt Gene Name: HSP90AB1
• UniProt Synonym Gene Names: HSP90B; HSPC2; HSPCB; HSP 90; HSP 84; HSP84
• UniProt Entry Name: HS90B_HUMAN

NCBI Description

This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012]

Uniprot Description

HSP90B: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4. Belongs to the heat shock protein 90 family.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: nucleoplasm; signalosome; cell surface; membrane; mitochondrion; brush border membrane; basolateral plasma membrane; cytoplasm; apical plasma membrane; melanosome; inclusion body; cytosol

Molecular Function: protein binding; GTP binding; TPR domain binding; nitric-oxide synthase regulator activity; double-stranded RNA binding; unfolded protein binding; dATP binding; sulfonylurea receptor binding; protein kinase binding; kinase binding; ATP binding

Biological Process: axon guidance; positive regulation of nitric oxide biosynthetic process; protein folding; positive regulation of protein import into nucleus, translocation; positive regulation of cell size; positive regulation of protein binding; innate immune response; negative regulation of neuron apoptosis; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; response to salt stress; response to unfolded protein; placenta development