Rabbit Rab5 Polyclonal Antibody | anti-RAB5A antibody

Rab5 Antibody

Cat. Num. AAA805447

• Gene Names: RAB5A; RAB5
• Reactivity: Human, Mouse, Monkey, Bovine, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry
• Synonyms: Rab5; Polyclonal Antibody; Rab5 Antibody; Rab 5A; RAS associated protein RAB5A; Ras related protein Rab 5 A; anti-RAB5A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Monkey, Bovine, Rat
• Clonality: Polyclonal
• Form/Format: Affinity Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 215

• Applicable Applications for anti-RAB5A antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: 1:1000 (WB), 1:150 (ICC)
• Certificate of Analysis: 1 uL/mL of SPC-168 was sufficient for detection of Rab5 in 15ug of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western blot analysis of Human Cell line lysates showing detection of Rab5 protein using Rabbit Anti-Rab5 Polyclonal Antibody (SPC-168). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody (SPC-168) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-Rab5 Polyclonal Antibody (SPC-168). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody (SPC-168) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.)

Related Product Information for anti-RAB5A antibody

Background Info: The antibody recognizes 26kDa Rab5 of the above species origins.

Scientific Background: Rab5 is a 24kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper variable COHO-terminal domains with a cysteine motif implicated in subcellular targeting. Post-translational modification of the cysteine motif with one or two geranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins(3). Each Rab shows a characteristic subcellular distribution (4). In particular, Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes (5). Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and EEA1 (6). Anti-Rab5 may be used as an early endosome marker.

Product Categories/Family for anti-RAB5A antibody

Cell Signaling; Neuroscience; Organelle Markers

References

1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7. 2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208. 3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412. 4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117. 5. Sonnichsen B., et al. (2000) J. Cell Biol. 149: 901-913 6. Woodman P.G. (2000) Traffic. 1: 695-701.
1. Ballmer-Hofer, K., Andersson, A.E., Ratcliffe, L.E. and Berger, P. (2011). Neuropilin-1 promotes VEGFR-2 trafficking through Rab11 vesicles thereby specifying signal output. Blood. 118 (3), 816-826. doi: 10.1182/blood-2011-01-328773.

NCBI and Uniprot Product Information

• NCBI GI #: 19923262
• NCBI GeneID: 5868
• NCBI Accession #: NP_004153.2
• NCBI GenBank Nucleotide #: NM_004162.4
• UniProt Accession #: Q6FI44; B4DJA5; P20339
• Molecular Weight: 22,178 Da
• NCBI Official Full Name: ras-related protein Rab-5A isoform 1
• NCBI Official Synonym Full Names: RAB5A, member RAS oncogene family
• NCBI Official Symbol: RAB5A
• NCBI Official Synonym Symbols: RAB5
• NCBI Protein Information: ras-related protein Rab-5A; RAS-associated protein RAB5A
• UniProt Protein Name: Ras-related protein Rab-5A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB5A
• UniProt Synonym Gene Names: RAB5
• UniProt Entry Name: RAB5A_HUMAN

Uniprot Description

RAB5A: Required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, RABEP1, SUN2, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3. Interacts with PIK3CB. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. Belongs to the small GTPase superfamily. Rab family.

Protein type: Motility/polarity/chemotaxis; G protein; G protein, monomeric, Rab; G protein, monomeric

Chromosomal Location of Human Ortholog: 3p24-p22

Cellular Component: zymogen granule membrane; protein complex; early endosome; dendrite; terminal button; phagocytic vesicle; cytosol; lipid raft; actin cytoskeleton; ruffle; recycling endosome; synaptic vesicle; cell soma; perinuclear region of cytoplasm; endocytic vesicle; axon; cytoplasm; plasma membrane; melanosome; endosome membrane; nerve terminal; endosome

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; GDP-dissociation inhibitor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; intracellular protein transport; regulation of endosome size; early endosome to late endosome transport; positive regulation of smooth muscle cell proliferation; regulation of endocytosis; endocytosis; regulation of filopodium formation; blood coagulation; positive regulation of smooth muscle cell migration; Rab protein signal transduction; positive regulation of exocytosis