Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10. Tissue: NRK cells. Species: Rat. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. KR-10 staining red; DAPI staining blue. Merged images. Courtesy of: Institute of Mol. and Cell Bio, Singapore.)
Mouse KDEL Monoclonal Antibody | anti-Kdelr1 antibody
KDEL Receptor Antibody: Alkaline Phosphatase
Gene Names
Kdelr1; Erd2.1; AW215843; 8030486F04Rik
Reactivity
Human, Monkey, Rat, Mouse, Hamster, Rabbit, Pig, Bovine, Sheep, Canine, Chicken, Drosophilia, Xenopus
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Immunofluorescence
Synonyms
KDEL; Monoclonal Antibody; KDEL Receptor Antibody: Alkaline Phosphatase; ERD2; ERD2.1; ERD21; HDEL; KDEL R1; KDELR1; PM23; anti-Kdelr1 antibody
Host
Mouse
Reactivity
Human, Monkey, Rat, Mouse, Hamster, Rabbit, Pig, Bovine, Sheep, Canine, Chicken, Drosophilia, Xenopus
Clonality
Monoclonal
Isotype
IgG1
Clone Number
KR-10
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
212
Applicable Applications for anti-Kdelr1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
1:1000 dilution was sufficient for detection by colorimetric analysis
Conjugate
Alkaline Phosphatase
Storage Buffer
PBS pH7.2, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10. Tissue: NRK cells. Species: Rat. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. KR-10 staining red; DAPI staining blue. Merged images. Courtesy of: Institute of Mol. and Cell Bio, Singapore.)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10. Tissue: NRK cells. Species: Rat. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. KR-10 staining red; DAPI staining blue. Merged images. Courtesy of: Institute of Mol. and Cell Bio, Singapore.)
Western Blot (WB)
(Western Blot analysis of Rat tissue lysate showing detection of KDEL Receptor protein using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Western Blot (WB)
(Western Blot analysis of Rat tissue lysate showing detection of KDEL Receptor protein using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Related Product Information for anti-Kdelr1 antibody
Background Info: Detects an ~25kDa protein corresponding to the molecular mass of KDEL on SDS-PAGE immunoblots.
Scientific Background: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity; and all are located within the cis-Golgi and its intermediate compartments (4). In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).
Scientific Background: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity; and all are located within the cis-Golgi and its intermediate compartments (4). In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).
Product Categories/Family for anti-Kdelr1 antibody
References
1. Whiteman P., and Handford P.A. (2003) Hum Mol Genet 12(7): 727-737. 2. Forthoffer N., et al. (2002) J Bioenerg Biomemb 34(3): 209-219. 3. Aoe T., et al. (1997) EMBO J. 16: 7305-7316. 4. Tang B.L., Wong S.H, Qi X.L. Low S.H., and Hong W. (1993) J. Cell Biol. 120: 325-328. 5. Lewis M.J. and Pelham H.R. (1990) Nature 348: 162-163. 6. Spurger L. (2002). Endoplasmic reticulum: Structure and function. University of Texas Medical Branch. Retrieved September 13, 2006, from http://cellbio.utmb.edu/cellbio/rer1.htm
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,560 Da
NCBI Official Full Name
ER lumen protein-retaining receptor 1
NCBI Official Synonym Full Names
KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1
NCBI Official Symbol
Kdelr1
NCBI Official Synonym Symbols
Erd2.1; AW215843; 8030486F04Rik
NCBI Protein Information
ER lumen protein-retaining receptor 1; KDEL receptor 1; ER lumen protein retaining receptor 1; KDEL endoplasmic reticulum protein retention receptor 1
UniProt Protein Name
ER lumen protein-retaining receptor 1
Protein Family
UniProt Gene Name
Kdelr1
UniProt Synonym Gene Names
KDEL receptor 1
UniProt Entry Name
ERD21_MOUSE
Uniprot Description
KDELR1: Required for the retention of luminal endoplasmic reticulum proteins. Determines the specificity of the luminal ER protein retention system. Also required for normal vesicular traffic through the Golgi. This receptor recognizes K-D-E-L. Belongs to the ERD2 family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Endoplasmic reticulum
Cellular Component: Golgi apparatus; cis-Golgi network; membrane; endoplasmic reticulum; ER-Golgi intermediate compartment; integral to membrane; cytoplasmic vesicle
Molecular Function: ER retention sequence binding
Biological Process: vesicle-mediated transport; protein transport; transport; protein retention in ER
Research Articles on Kdelr1
Similar Products
Product Notes
The Kdelr1 kdelr1 (Catalog #AAA802511) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KDEL Receptor Antibody: Alkaline Phosphatase reacts with Human, Monkey, Rat, Mouse, Hamster, Rabbit, Pig, Bovine, Sheep, Canine, Chicken, Drosophilia, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's KDEL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF). 1:1000 dilution was sufficient for detection by colorimetric analysis. Researchers should empirically determine the suitability of the Kdelr1 kdelr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KDEL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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