Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.)
Mouse SUR1 Monoclonal Antibody | anti-Abcc8 antibody
SUR1 Antibody: RPE
Gene Names
Abcc8; Sur; Sur1
Reactivity
Mouse, Hamster, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Synonyms
SUR1; Monoclonal Antibody; SUR1 Antibody: RPE; AM60008PU-N; ABC36; Abcc8; ATP binding cassette sub family C member 8; HHF1; HRINS; MRP8; PHHI; SUR; Sulfonylurea receptor (hyperinsulinemia); ATP binding cassette sub family C (CFTR/MRP) member 8; anti-Abcc8 antibody
Host
Mouse
Reactivity
Mouse, Hamster, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S289-16
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
1582
Applicable Applications for anti-Abcc8 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
1ug/mL (WB), 0.1-1ug/mL (IHC)
Conjugate
RPE
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.)
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.)
Related Product Information for anti-Abcc8 antibody
Background Info: Detects ~160kDa. Does not cross-react with SUR2B
Scientific Background: Sulfonylurea receptors (SUR) are membrane proteins which are the molecular targets of the sulfonylurea class of anti-diabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. More specifically, SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2) (1). The association of four Kir6.x and four SUR subunits form an ion conducting channel commonly referred to as the KATP channel. The primary function of the sulfonylurea receptor is to sense intracellular levels of the nucleotides ATP and ADP and in response facilitate the open or closing its associated Kir6.x potassium channel. Hence the KATP channel monitors the energy balance within the cell (2).
Scientific Background: Sulfonylurea receptors (SUR) are membrane proteins which are the molecular targets of the sulfonylurea class of anti-diabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. More specifically, SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2) (1). The association of four Kir6.x and four SUR subunits form an ion conducting channel commonly referred to as the KATP channel. The primary function of the sulfonylurea receptor is to sense intracellular levels of the nucleotides ATP and ADP and in response facilitate the open or closing its associated Kir6.x potassium channel. Hence the KATP channel monitors the energy balance within the cell (2).
Product Categories/Family for anti-Abcc8 antibody
References
1. Campbell J.D., Sansom M.S., Ashcroft F.M. (2003) EMBO Resp. 4(11): 1038-1042. 2. Nichols C.G. (2006) Nature. 440 (7083): 470-476.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
177,097 Da
NCBI Official Full Name
ATP-binding cassette sub-family C member 8
NCBI Official Synonym Full Names
ATP-binding cassette, subfamily C (CFTR/MRP), member 8
NCBI Official Symbol
Abcc8
NCBI Official Synonym Symbols
Sur; Sur1
NCBI Protein Information
ATP-binding cassette sub-family C member 8; sulfonylurea receptor 1; sulphonylurea receptor 1; ATP-binding cassette transporter sub-family C member 8; ATP-binding cassette, sub-family C (CFTR/MRP), member 8
UniProt Protein Name
ATP-binding cassette sub-family C member 8
Protein Family
UniProt Gene Name
Abcc8
UniProt Synonym Gene Names
Sur; Sur1
UniProt Entry Name
ABCC8_RAT
NCBI Description
binds sulfonylurea; subunit of the ATP-sensitive potassium channel K(ATP), which is composed of Sur1 and the inwardly rectifying K(+) channel subunit, Kir6.2 [RGD, Feb 2006]
Uniprot Description
Subunit of the beta-cell ATP-sensitive potassium channel (KATP). Regulator of ATP-sensitive K+ channels and insulin release.
Research Articles on Abcc8
Similar Products
Product Notes
The Abcc8 abcc8 (Catalog #AAA800985) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SUR1 Antibody: RPE reacts with Mouse, Hamster, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SUR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). 1ug/mL (WB), 0.1-1ug/mL (IHC). Researchers should empirically determine the suitability of the Abcc8 abcc8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SUR1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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