Mouse SUR1 Monoclonal Antibody | anti-Abcc8 antibody

SUR1 Antibody: ATTO 488

Cat. Num. AAA802683

• Gene Names: Abcc8; Sur; Sur1
• Reactivity: Human, Mouse, Hamster, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein G Purified
• Synonyms: SUR1; Monoclonal Antibody; SUR1 Antibody: ATTO 488; AM60008PU-N; ABC36; Abcc8; ATP binding cassette sub family C member 8; HHF1; HRINS; MRP8; PHHI; SUR; Sulfonylurea receptor (hyperinsulinemia); ATP binding cassette sub family C (CFTR/MRP) member 8; anti-Abcc8 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Hamster, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S289-16
• Specificity: Detects ~160kDa. Does not cross-react with SUR2B.
• Purity/Purification: Protein G Purified
• Form/Format: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 1582

• Applicable Applications for anti-Abcc8 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB (1:1000); IHC (1:1000); ICC/IF (1:100); ; Optimal dilutions for assays should be determined by the user.
• Cellular Localization: Membrane
• Immunogen: Fusion protein amino acids 1548-1582 (cytoplasmic C-terminus) of rat SUR1.
• Certificate of Analysis: 1 ug/ml of MBS802683 was sufficient for detection of SUR1 in 20 ug of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Research Areas: Atherosclerosis, Cancer, Cardiovascular System, Cell Markers, Cell Signaling, Heart, Membrane Markers, Neuron Markers, Neuroscience
• Preparation and Storage: Store at -20°C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (MBS802683). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (MBS802683) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.)

Immunocytochemistry/Immunofluorescence(ICC/IF)

( Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (MBS802683). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (MBS802683) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SUR1 Antibody. (D) Composite.)

Related Product Information for anti-Abcc8 antibody

Sulfonylurea receptors (SUR) are membrane proteins which are the molecular targets of the sulfonylurea class of anti-diabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. More specifically, SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2) (1). The association of four Kir6.x and four SUR subunits form an ion conducting channel commonly referred to as the KATP channel. The primary function of the sulfonylurea receptor is to sense intracellular levels of the nucleotides ATP and ADP and in response facilitate the open or closing its associated Kir6.x potassium channel. Hence the KATP channel monitors the energy balance within the cell (2).

Product Categories/Family for anti-Abcc8 antibody

Cell Signaling

References

1.   Campbell J.D., Sansom M.S., Ashcroft F.M. (2003) EMBO Resp. 4(11): 1038-1042. 2.   Nichols C.G. (2006) Nature. 440 (7083): 470-476.

NCBI and Uniprot Product Information

• NCBI GI #: 148368981
• NCBI GeneID: 25559
• NCBI Accession #: NP_037171.2
• NCBI GenBank Nucleotide #: NM_013039.2
• UniProt Accession #: Q09429; O54989; P70532; Q9EQT0
• NCBI Official Full Name: ATP-binding cassette sub-family C member 8
• NCBI Official Synonym Full Names: ATP-binding cassette, subfamily C (CFTR/MRP), member 8
• NCBI Official Symbol: Abcc8
• NCBI Official Synonym Symbols: Sur; Sur1
• NCBI Protein Information: ATP-binding cassette sub-family C member 8; sulfonylurea receptor 1; sulphonylurea receptor 1; ATP-binding cassette transporter sub-family C member 8; ATP-binding cassette, sub-family C (CFTR/MRP), member 8
• UniProt Protein Name: ATP-binding cassette sub-family C member 8
• Protein Family: ABC transporter C family
• UniProt Gene Name: Abcc8
• UniProt Synonym Gene Names: Sur; Sur1
• UniProt Entry Name: ABCC8_RAT

NCBI Description

binds sulfonylurea; subunit of the ATP-sensitive potassium channel K(ATP), which is composed of Sur1 and the inwardly rectifying K(+) channel subunit, Kir6.2 [RGD, Feb 2006]

Uniprot Description

Subunit of the beta-cell ATP-sensitive potassium channel (KATP). Regulator of ATP-sensitive K+ channels and insulin release.