Mouse HIF1 alpha Monoclonal Antibody | anti-HIF1 alpha antibody

HIF1 alpha Antibody

Cat. Num. AAA8001935

• Gene Names: Hif1a; MOP1; bHLHe78; AA959795; HIF1alpha; HIF1-alpha; HIF-1-alpha
• Reactivity: Human, Mouse, Rat, Bovine
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, ELISA
• Purity: Protein G Purified
• Synonyms: HIF1 alpha; Monoclonal Antibody; HIF1 alpha Antibody; Mouse Anti-Mouse HIF1 alpha Monoclonal IgG1; HIF 1 alpha Antibody; Clone ESEE122; ARNT interacting protein Antibody; HIF1A Antibody; Hypoxia inducible factor 1 alpha Antibody; MOP1 Antibody; PASD8 Antibody; anti-HIF1 alpha antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Bovine
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: ESEE122
• Specificity: Detects ~116kDa. Specific for HIF1Alpha.
• Purity/Purification: Protein G Purified
• Form/Format: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 837

• Applicable Applications for anti-HIF1 alpha antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:1000; IHC: 1:100; ICC/IF: 1:50
• Immunogen: Recombinant fragment corresponding to amino acids 329-530
• Immunogen Species: Mouse
• Conjugate: Unconjugated
• Cellular Localization: Cytoplasm, Nucleus
• Certificate of Analysis: 1 ug/ml of SMC-184 was sufficient for detection of HIF1alpha in 20 ug of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Preparation and Storage: Store at-20 degree C.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-HIF1 alpha Monoclonal Antibody, Clone ESEE122 . Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HIF1 alpha Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Membranous and cytoplasmic localization in the epidermis, positive hair follicles, muscle and dermis.)

Western Blot (WB)

(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of HIF1 alpha protein using Mouse Anti-HIF1 alpha Monoclonal Antibody, Clone ESEE122 . Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HIF1 alpha Monoclonal Antibody at 1:500 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Related Product Information for anti-HIF1 alpha antibody

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1alpha is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1alpha is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1alpha degradation and lead to its stabilization. In addition, HIF-1alpha can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).

Product Categories/Family for anti-HIF1 alpha antibody

Cancer; Oxidative Stress; Cell Signaling; Epigenetics and Nuclear Signaling

References

1. Sharp F.R. and Bernaudin M. (2004) Nat Rev Neurosci 5: 437-48. 2. Wang G.L., et al. (1995) Proc Natl Acad Sci U S A 92: 5510-4. 3. Jaakkola P., et al. (2001) Science 292: 468-72. 4. Maxwell P.H., et al. (1999) Nature 399: 271-5. 5. Fukuda R., et al. (2002) J Biol Chem 277: 38205-11. 6. Jiang B.H., et al. (2001) Cell Growth Differ 12: 363-9. 7. Laughner E., et al. (2001) Mol Cell Biol 21: 3995-4004.

NCBI and Uniprot Product Information

• NCBI GI #: 927028881
• NCBI GeneID: 15251
• NCBI Accession #: NP_001300848.1
• NCBI GenBank Nucleotide #: NM_001313919.1
• UniProt Accession #: Q61221
• NCBI Official Full Name: hypoxia-inducible factor 1-alpha isoform 1
• NCBI Official Synonym Full Names: hypoxia inducible factor 1, alpha subunit
• NCBI Official Symbol: Hif1a
• NCBI Official Synonym Symbols: MOP1; bHLHe78; AA959795; HIF1alpha; HIF1-alpha; HIF-1-alpha
• NCBI Protein Information: hypoxia-inducible factor 1-alpha
• UniProt Protein Name: Hypoxia-inducible factor 1-alpha
• UniProt Gene Name: Hif1a
• UniProt Synonym Gene Names: HIF-1-alpha; HIF1-alpha

NCBI Description

This gene encodes the alpha subunit which, along with the beta subunit, forms a heterodimeric transcription factor that regulates the cellular and developmental response to reduced oxygen tension. The transcription factor has been shown to regulate genes involved in several biological processes, including erythropoiesis and angiogenesis which aid in increased delivery of oxygen to hypoxic regions. The transcription factor also plays a role in the induction of genes involved in cell proliferation and survival, energy metabolism, apoptosis, and glucose and iron metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia ().