Rabbit anti-Human CBP Polyclonal Antibody | anti-CBP antibody

CBP Antibody

Cat. Num. AAA8000949

• Gene Names: CREBBP; CBP; RSTS; KAT3A; RSTS1
• Reactivity: Human
• Applications: Western Blot
• Purity: Peptide Affinity Purified
• Synonyms: CBP; Polyclonal Antibody; CBP Antibody; Rabbit Anti-Human CBP Polyclonal; RSTS Antibody; CBP_HUMAN Antibody; Cyclic AMP responsive enhancer binding protein Antibody; Crebbp Antibody; CREB-binding protein Antibody; Rubinstein Taybi syndrome Antibody; KAT3A Antibody; EC:2.3.1.48 Antibody; CREBBP Antibody; RTS Antibody; CREB binding protein Antibody; anti-CBP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Detects 265 kDa.
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 2404

• Applicable Applications for anti-CBP antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Target: CBP
• Immunogen: Synthetic peptide from the mid-protein of Human CBP (aa. 1000-1100)
• Immunogen Species: Human
• Cellular Localization: Cytoplasm; Nucleus
• Conjugate: Unconjugated
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-751). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-751) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)

Product Categories/Family for anti-CBP antibody

Antibodies; Cell Signaling; Epigenetics and Nuclear Signaling; Chromatin Modifying Enzymes; Transcription; Stem Cells; Signaling Pathways; Wnt/Beta Catenin Pathway; Cancer; Cancer Metabolism; Cancer Lipid and Lipoprotein Metabolism; Cancer; Cancer Metab

NCBI and Uniprot Product Information

• NCBI GI #: 119943102
• NCBI GeneID: 1387
• NCBI Accession #: NP_001073315.1
• NCBI GenBank Nucleotide #: NM_001079846.1
• UniProt Accession #: Q92793; O00147; Q16376; Q4LE28; D3DUC9
• Molecular Weight: 260,993 Da
• NCBI Official Full Name: CREB-binding protein isoform b
• NCBI Official Synonym Full Names: CREB binding protein
• NCBI Official Symbol: CREBBP
• NCBI Official Synonym Symbols: CBP; RSTS; KAT3A; RSTS1
• NCBI Protein Information: CREB-binding protein
• UniProt Protein Name: CREB-binding protein
• Protein Family: CBP3-like protein
• UniProt Gene Name: CREBBP
• UniProt Synonym Gene Names: CBP

NCBI Description

This gene is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving this gene have been associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]

Uniprot Description

Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 and FOXO1. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902).