Rabbit CYP1A2 Polyclonal Antibody | anti-CYP1A2 antibody

CYP1A2-Specific Rabbit Polyclonal

Cat. Num. AAA768380

• Gene Names: CYP1A2; CP12; P3-450; P450(PA)
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: CYP1A2; Polyclonal Antibody; CYP1A2-Specific Rabbit Polyclonal; anti-CYP1A2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 516

• Applicable Applications for anti-CYP1A2 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Immunogen: Cytochrome P450, family 1, subfamily A, polypeptide 2
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver tissue slide using MBS768380 (CYP1A2-Specific Antibody) at dilution of 1:200)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells using MBS768380 (CYP1A2 antibody) at dilution of 1:25 and Rhodamine-conjugated Goat Anti-Rabbit IgG (H+L))

Immunoprecipitation (IP)

(IP result of CYP1A2-Specific antibody (IP: MBS768380,4ug; Detection: MBS768380 1:300) with mouse liver tissue 3000ug.)

Western Blot (WB)

(mouse liver tissue were subjected to SDS PAGE followed by western blot with MBS768380 (CYP1A2-Specific antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 73915100
• NCBI GeneID: 1544
• NCBI Accession #: NP_000752.2
• NCBI GenBank Nucleotide #: NM_000761.4
• Molecular Weight: 58,407 Da
• NCBI Official Full Name: cytochrome P450 1A2
• NCBI Official Synonym Full Names: cytochrome P450 family 1 subfamily A member 2
• NCBI Official Symbol: CYP1A2
• NCBI Official Synonym Symbols: CP12; P3-450; P450(PA)
• NCBI Protein Information: cytochrome P450 1A2
• UniProt Protein Name: Cytochrome P450 1A2
• Protein Family: Cytochrome
• UniProt Gene Name: CYP1A2
• UniProt Entry Name: CP1A2_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP1A2: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O- deethylation of phenacetin. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - linoleic acid; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Oxidoreductase; Amino Acid Metabolism - tryptophan; Secondary Metabolites Metabolism - caffeine; Cofactor and Vitamin Metabolism - retinol; EC 1.14.14.1; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function: demethylase activity; electron carrier activity; enzyme binding; heme binding; iron ion binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding

Biological Process: alkaloid metabolic process; cellular respiration; dibenzo-p-dioxin metabolic process; drug catabolic process; drug metabolic process; epoxygenase P450 pathway; exogenous drug catabolic process; heterocycle metabolic process; hydrogen peroxide biosynthetic process; lung development; methylation; monocarboxylic acid metabolic process; monoterpenoid metabolic process; porphyrin metabolic process; post-embryonic development; regulation of gene expression; response to estradiol stimulus; response to lipopolysaccharide; steroid catabolic process; toxin biosynthetic process; xenobiotic metabolic process