Human Properdin ELISA Kit | CFP elisa kit

Human Properdin ELISA Kit

Cat. Num. AAA765961

• Gene Names: CFP; BFD; PFC; PFD; PROPERDIN
• Reactivity: Human
• Synonyms: Properdin; Human Properdin ELISA Kit; CFP/Complement Factor P/Properdin/PFC/BFD/complement factor properdin/PFCcomplement; CFP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CFP. No significant cross-reactivity or interference between CFP and analogues was observed.
• Sequence Length: 469

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 1.563-100ng/ml
• Sensitivity: 0.938ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CFP elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 223671861
• NCBI GeneID: 5199
• NCBI Accession #: NP_001138724.1
• NCBI GenBank Nucleotide #: NM_001145252.1
• Molecular Weight: 51,276 Da
• NCBI Official Full Name: properdin
• NCBI Official Synonym Full Names: complement factor properdin
• NCBI Official Symbol: CFP
• NCBI Official Synonym Symbols: BFD; PFC; PFD; PROPERDIN
• NCBI Protein Information: properdin
• UniProt Protein Name: Properdin
• Protein Family: Properdin
• UniProt Gene Name: CFP
• UniProt Synonym Gene Names: PFC
• UniProt Entry Name: PROP_HUMAN

NCBI Description

This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Feb 2009]

Uniprot Description

CFP: A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Defects in CFP are the cause of properdin deficiency (PFD). PFD results in higher susceptibility to bacterial infections; especially to meningococcal infections. Three phenotypes have been reported: complete deficiency (type I), incomplete deficiency (type II), and dysfunction of properdin (type III).

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: Xp11.4

Cellular Component: endoplasmic reticulum lumen; extracellular matrix; extracellular region; extracellular space

Biological Process: cellular protein metabolic process; complement activation; complement activation, alternative pathway; defense response to bacterium; immune response; innate immune response; post-translational protein modification; protein amino acid O-linked glycosylation; regulation of complement activation

Disease: Properdin Deficiency, X-linked